ATTO 532
Optische Eigenschaften |
||||
λabs |
532 |
nm |
||
εmax |
1,15×105 |
M-1 cm-1 |
||
λfl |
552 |
nm |
||
ηfl |
90 |
% |
||
τfl |
3,8 |
ns |
||
CF260 = ε260/εmax |
0,20 |
|
||
CF280 = ε280/εmax |
0,09 |
|
ATTO 532 ist ein Fluoreszenzmarker mit hervorragender Wasserlöslichkeit. Zu den charakteristischen Eigenschaften des Farbstoffs zählen starke Absorption, sehr hohe Fluoreszenzquantenausbeute sowie hohe thermische und photochemische Stabilität. Der Farbstoff ist somit besonders geeignet im Bereich der Einzelmoleküldetektion sowie zur hochauflösenden Mikroskopie (SIM, STED, etc.). Eine effiziente Anregung der Fluoreszenz erfolgt im Bereich 515 - 545 nm. Eine für den Farbstoff geeignete Anregungslichtquelle ist der frequenzverdoppelte Nd:YAG-Laser mit seiner Linie bei 532 nm.
Produktdatenblätter
- ATTO 532 Produktinformation
- NHS-Ester
- Maleimid
- Iodacetamid
- Phalloidin
- Amin
- Azid/Alkin
- Streptavidin
- Tetrazin (MeTet)
Absorptions- und Emissionsspektrum (ASCII)
- ATTO 532 Absorption (.txt)
- ATTO 532 Emission (.txt)
Absorptions- und Emissionsspektrum (Grafik)
- ATTO 532 Absorption/Fluoreszenz (.jpg)
Sicherheitsdatenblätter
- ATTO 532 Carboxy MSDS
- ATTO 532 NHS-Ester MSDS
- ATTO 532 Maleimid MSDS
- ATTO 532 Streptavidin MSDS
- ATTO 532 Biotin MSDS
- ATTO 532 Phalloidin MSDS
- ATTO 532 Amin MSDS
- ATTO 532 Azid MSDS
- ATTO 532 Iodacetamid MSDS
- ATTO 532 Hydrazid MSDS
- ATTO 532 Alkin MSDS
- ATTO 532 Cadaverin MSDS
- ATTO 532 MeTet MSDS
- ATTO 532 Peg(4)-DBCO MSDS
Ausgewählte Literatur zu ATTO 532:
G. Campagnola, O. Peersen, Co-folding and RNA activation of poliovirus 3Cpro polyprotein precursors, Journal of Biological Chemistry 299, 105258 (2023).
U. Günsel, K. Klöpfer, E. Häusler, M. Hitzenberger, B. Bölter, L. Sperl, M. Zacharias, J. Soll, F. Hagn, Structural basis of metabolite transport by the chloroplast outer envelope channel OEP21, Nature structural & molecular biology 30, 761 (2023).
S. Napoletano, E. Battista, N. Martone, P. Netti, F. Causa, Direct, precise, enzyme-free detection of miR-103–3p in real samples by microgels with highly specific molecular beacons, Talanta 259, 124468 (2023).
M. Schenkel, D. Ravamehr-Lake, T. Czerniak, J. Saenz, G. Krainer, M. Schlierf, C. Deber, Impact of cholesterol and Lumacaftor on the folding of CFTR helical hairpins, Biochimica et Biophysica Acta (BBA) - Biomembranes 1865, 184078 (2023).
M. Vogt, J. List, M. Langecker, I. Santiago, F. Simmel, E. Kopperger, Electrokinetic Torque Generation by DNA Nanorobotic Arms Studied via Single-Molecule Fluctuation Analysis, The Journal of Physical Chemistry B 127, 10710 (2023).
G. Yakoub, Y.-S. Choi, R. Wong, T. Strauch, K. Ann, R. Cohen, H. Ulrich, Avidity-based biosensors for ubiquitylated PCNA reveal choreography of DNA damage bypass, Science advances 9, eadf3041 (2023).
O. Abdelraouf, Z. Wang, H. Liu, Z. Dong, Q. Wang, M. Ye, X. Wang, Q. Wang, H. Liu, Recent Advances in Tunable Metasurfaces, ACS Nano 16, 13339 (2022).
G. Agam, C. Gebhardt, et al., Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins, Nature Methods, 1 (2023).
M. Bespalova, A. Behjatian, N. Karedla, R. Walker-Gibbons, M. Krishnan, Opto-Electrostatic Determination of Nucleic Acid Double-Helix Dimensions and the Structure of the Molecule-Solvent Interface, Macromolecules 55, 6200 (2022).
M. Bespalova, R. Öz, F. Westerlund, M. Krishnan, Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System, Langmuir : the ACS journal of surfaces and colloids 38, 13923 (2022).
K. Hübner, M. Raab, J. Bohlen, J. Bauer, P. Tinnefeld, Salt-induced conformational switching of a flat rectangular DNA origami structure, Nanoscale 14, 7898 (2022).
G. Kloes, T. Bennett, A. Chapet-Batlle, A. Behjatian, A. Turberfield, M. Krishnan, Far-Field Electrostatic Signatures of Macromolecular 3D Conformation, Nano letters 22, 7834 (2022).
J. Langeland, T. Lindkvist, C. Kjær, S. Nielsen, Gas-phase Förster resonance energy transfer in mass-selected and trapped ions, Mass Spectrometry Reviews n/a, e21828 (2022).
D. Niranjan Dhanasekar, D. Thiyagarajan, D. Bhatia, DNA Origami in the Quest for Membrane Piercing, Chemistry – An Asian Journal 17, e202200591 (2022).
M. Polesel, M. Kaminska, D. Haenni, M. Bugarski, C. Schuh, N. Jankovic, A. Kaech, J. Mateos, M. Berquez, A. Hall, Spatiotemporal organisation of protein processing in the kidney, Nature Communications 13, 1 (2022).
G. Rathod, D. Boyle, J. Amamcharla, Acid gelation properties of fibrillated model milk protein concentrate dispersions, Journal of Dairy Science 105, 4925 (2022).
M. Safar, A. Saurabh, B. Sarkar, M. Fazel, K. Ishii, T. Tahara, I. Sgouralis, S. Pressé, Single-photon smFRET. III. Application to pulsed illumination, Biophysical Reports 2, 100088 (2022).
C. Schnupfhagn, T. Schumacher, P. Markus, G. Papastavrou, O. Aftenieva, T. König, V. Dudko, M. Matejdes, J. Breu, M. Lippitz, Disentangling the Orientations of Spectrally Overlapping Transition Dipoles in Dense Dye Layers, Nano letters 22, 7499 (2022).
S. Shanmugam, S. Trashin, K. de Wael, Singlet oxygen-based photoelectrochemical detection of DNA, Biosensors and Bioelectronics 195, 113652 (2022).
E. Thomas, C. Cortes, L. Paul, S. Gray, K. Thomas, Combined effects of emitter-emitter and emitter-plasmonic surface separations dictate photoluminescence enhancement in a plasmonic field, Physical chemistry chemical physics : PCCP 24, 17250 (2022).
C. Amarasekara, C. Rathnayaka, U. Athapattu, L. Zhang, J. Choi, S. Park, A. Nagel, S. Soper, Electrokinetic identification of ribonucleotide monophosphates (rNMPs) using thermoplastic nanochannels, Journal of Chromatography A 1638, 461892 (2021).
G. Berselli, A. Gimenez, A. O’Connor, T. Keyes, Robust Photoelectric Biomolecular Switch at a Microcavity-Supported Lipid Bilayer, ACS Applied Materials & Interfaces 13, 29158 (2021).
K. Chen, R. Yan, L. Xiang, K. Xu, Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing, Light: Science & Applications 10, 1 (2021).
L. Doll, J. Lackner, F. Rönicke, G. Nienhaus, H.-A. Wagenknecht, Fluorescence Lifetime Imaging Microscopy (FLIM) of Intracellular Transport by Means of Doubly Labelled siRNA Architectures, ChemBioChem 22, 2561 (2021).
K. Kho, G. Berselli, T. Keyes, A Nanoplasmonic Assay of Oligonucleotide-Cargo Delivery from Cationic Lipoplexes, Small 17, 2005815 (2021).
I. König, A. Soranno, D. Nettels, B. Schuler, Impact of In-Cell and In-Vitro Crowding on the Conformations and Dynamics of an Intrinsically Disordered Protein, Angewandte Chemie 133, 10819 (2021).
L. Masullo, F. Steiner, J. Zähringer, L. Lopez, J. Bohlen, L. Richter, F. Cole, P. Tinnefeld, F. Stefani, Pulsed Interleaved MINFLUX, Nano letters 21, 840 (2021).
E. Perego, S. Köster, Exploring early time points of vimentin assembly in flow by fluorescence fluctuation spectroscopy, Lab on a Chip 21, 735 (2021).
M. Pfeiffer, K. Trofymchuk, S. Ranallo, F. Ricci, F. Steiner, F. Cole, V. Glembockyte, P. Tinnefeld, Single antibody detection in a DNA origami nanoantenna, iScience 24, 103072 (2021).
I. Rasskazov, A. Moroz, P. Carney, Extraordinary Fluorescence Enhancement in Metal-Dielectric Core–Shell Nanoparticles, The Journal of Physical Chemistry Letters 12, 6425 (2021).
T. Rudack, C. Teuber, M. Scherlo, J. Güldenhaupt, J. Schartner, M. Lübben, J. Klare, K. Gerwert, C. Kötting, The Ras dimer structure, Chemical Science 12, 8178 (2021).
B. Schuler, I. König, A. Soranno, D. Nettels, Impact of in-cell and in-vitro crowding on the conformations and dynamics of an intrinsically disordered protein, Angewandte Chemie International Edition 60, 10724 (2021).
S. Suarasan, C. Tira, M. Rusu, A.-M. Craciun, M. Focsan, Controlled fluorescence manipulation by core-shell multilayer of spherical gold nanoparticles, Journal of Molecular Structure 1244, 130950 (2021).
A. Szalai, C. Zaza, F. Stefani, Super-resolution FRET measurements, Nanoscale 13, 18421 (2021).
R. Wu, J. Metternich, P. Tiwari, R. Zenobi, Adapting a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer for Gas-Phase Fluorescence Spectroscopy Measurement of Trapped Biomolecular Ions, Analytical Chemistry 93, 15626 (2021).
G. Cheng, J. Luo, Y. Liu, X. Chen, Z. Wu, T. Chen, Cucurbituril-Oriented Nanoplatforms in Biomedical Applications, ACS Applied Bio Materials 3, 8211 (2020).
M. Shah, D. Corbett, A. Lanzaro, A. Roche, N. Sibanda, P. Davis, S. Uddin, C. van der Walle, R. Curtis, A. Pluen, Micro- and macro-viscosity relations in high concentration antibody solutions, European Journal of Pharmaceutics and Biopharmaceutics 153, 211 (2020).
C. Amarasekara, U. Athapattu, C. Rathnayaka, J. Choi, S. Park, S. Soper, Open-tubular nanoelectrochromatography (OT-NEC), Encyclopedia of Marine Biotechnology 41, 1627 (2020).
J. Bartl, S. Gremmo, M. Stutzmann, M. Tornow, A. Cattani-Scholz, Modification of silicon nitride with oligo(ethylene glycol)-terminated organophosphonate monolayers, Surface Science 697, 121599 (2020).
M. Burger, S. Rein, S. Weber, P. Gräber, S. Kacprzak, Distance measurements in the F0F1-ATP synthase from E. coli using smFRET and PELDOR spectroscopy, European biophysics journal : EBJ 49, 1 (2020).
P. Gaur, M. Galkin, S. Hauke, R. Redkin, C. Barnes, V. Shvadchak, D. Yushchenko, Reversible spatial and temporal control of lipid signaling, Chemical Communications 56, 10646 (2020).
G. Krainer, A. Hartmann, V. Bogatyr, J. Nielsen, M. Schlierf, D. Otzen, SDS-induced multi-stage unfolding of a small globular protein through different denatured states revealed by single-molecule fluorescence, Chemical Science 11, 9141 (2020).
K. Kramm, T. Schröder, J. Gouge, A. Vera, K. Gupta, F. Heiss, T. Liedl, C. Engel, I. Berger, A. Vannini, P. Tinnefeld, D. Grohmann, DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability, Nature Communications 11, 2828 (2020).
S. Pritzl, P. Urban, A. Prasselsperger, D. Konrad, J. Frank, D. Trauner, T. Lohmüller, Photolipid Bilayer Permeability is Controlled by Transient Pore Formation, Langmuir 36, 13509 (2020).
B. Shen, P. Piskunen, S. Nummelin, Q. Liu, M. Kostiainen, V. Linko, Advanced DNA Nanopore Technologies, ACS Applied Bio Materials 3, 5606 (2020).
K. Winkler, A. Karner, A. Horner, C. Hannesschlaeger, D. Knyazev, C. Siligan, M. Zimmermann, R. Kuttner, P. Pohl, J. Preiner, Interaction of the motor protein SecA and the bacterial protein translocation channel SecYEG in the absence of ATP, Nanoscale Advances 2, 3431 (2020).
S. Burg, M. Cohen, M. Margulis, S. Roth, A. Danielli, Magnetically aggregated biosensors for sensitive detection of biomarkers at low concentrations, Applied Physics Letters 115, 103702 (2019).
Y.-S. Choi, S. Bollinger, L. Prada, F. Scavone, T. Yao, R. Cohen, High-affinity free ubiquitin sensors for quantifying ubiquitin homeostasis and deubiquitination, Nature Methods 16, 771 (2019).
P. Gaur, O. Kucherak, Y. Ermakova, V. Shvadchak, D. Yushchenko, Nitrobenzyl-based fluorescent photocages for spatial and temporal control of signalling lipids in cells, Chemical Communications 55, 12288 (2019).
M. Goetzfried, K. Vogele, A. Mückl, M. Kaiser, N. Holland, F. Simmel, T. Pirzer, Periodic Operation of a Dynamic DNA Origami Structure Utilizing the Hydrophilic–Hydrophobic Phase‐Transition of Stimulus‐Sensitive Polypeptides, Small 15 (2019).
H.-Y. Hsieh, Y.-H. Peng, S.-F. Lin, L.-C. Chen, T.-C. Yu, C.-F. Chiou, J. Lee, Triple-Junction Optoelectronic Sensor with Nanophotonic Layer Integration for Single-Molecule Level Decoding, ACS Nano 13, 4486 (2019).
J. Huang, A. Traverso, G. Yang, M. Mikkelsen, Real-Time Tunable Strong Coupling:, ACS Photonics 6, 838 (2019).
M. Mohammadi-Kambs, A. Ott, DNA oligomer binding in competition exhibits cooperativity, New Journal of Physics 21, 113027 (2019).
E. Özelci, B. Rühle, F. Weigert, B. Lubotzky, G. Kewes, U. Resch-Genger, O. Benson, Quantitative Measurements of the pH-Sensitive Quantum Yield of Fluorophores in Mesoporous Silica Thin Films Using a Drexhage-Type Experiment, The Journal of Physical Chemistry C 123, 20468 (2019).
J. Pospíšil, T. Lukes, J. Bendesky, K. Fliegel, K. Spendier, G. Hagen, Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction, GigaScience 8 (2019).
A.R. Sekhar, B. Mallik, V. Kumar, J. Sankar, A cell-permeant small molecule for the super-resolution imaging of the endoplasmic reticulum in live cells, Organic & Biomolecular Chemistry 17, 3732 (2019).
U. Stella, L. Boarino, N. de Leo, P. Munzert, E. Descrovi, Enhanced Directional Light Emission Assisted by Resonant Bloch Surface Waves in Circular Cavities, ACS Photonics 6, 2073 (2019).
S. Wen, J. Zhou, P. Schuck, Y. Doug Suh, T. Schmidt, D. Jin, Future and challenges for hybrid upconversion nanosystems, Nature Photonics 13, 828 (2019).
W. Wong, J.-Y. Juo, C.-H. Lin, Y.-H. Liao, C.-Y. Cheng, C.-L. Hsieh, Characterization of Single-Protein Dynamics in Polymer-Cushioned Lipid Bilayers Derived from Cell Plasma Membranes, The journal of physical chemistry. B 123, 6492 (2019).
V. Dahiya, G. Agam, J. Lawatscheck, D. Rutz, D. Lamb, J. Buchner, Coordinated Conformational Processing of the Tumor Suppressor Protein p53 by the Hsp70 and Hsp90 Chaperone Machineries, Molecular Cell 74, 816-830.e7 (2019).
C. Davis, M. Gruebele, Labeling for Quantitative Comparison of Imaging Measurements in Vitro and in Cells, Biochemistry 57, 1929 (2018).
N. Dekhil, M. Skhairia, B. Mhenni, S. Ben Fradj, R. Warren, H. Mardassi, Automated IS6110-based fingerprinting of Mycobacterium tuberculosis, PLOS ONE 13, e0197913 (2018).
E. Frotscher, G. Krainer, A. Hartmann, M. Schlierf, S. Keller, Conformational Dynamics Govern the Free-Energy Landscape of a Membrane-Interacting Protein, ACS Omega 3, 12026 (2018).
D. Hammler, A. Marx, A. Zumbusch, Fluorescence-Lifetime-Sensitive Probes for Monitoring ATP Cleavage, Chemistry (Weinheim an der Bergstrasse, Germany) 24, 15329 (2018).
E. Hemmig, C. Fitzgerald, C. Maffeo, L. Hecker, S. Ochmann, A. Aksimentiev, P. Tinnefeld, U. Keyser, Optical Voltage Sensing Using DNA Origami, Nano letters 18, 1962 (2018).
G. Krainer, A. Treff, A. Hartmann, T. Stone, M. Schenkel, S. Keller, C. Deber, M. Schlierf, A minimal helical-hairpin motif provides molecular-level insights into misfolding and pharmacological rescue of CFTR, Communications Biology 1, 154 (2018).
F. Neubert, G. Beliu, U. Terpitz, C. Werner, C. Geis, M. Sauer, S. Doose, Bioorthogonal Click Chemistry Enables Site-specific Fluorescence Labeling of Functional NMDA Receptors for Super-Resolution Imaging, Angewandte Chemie International Edition 57, 16364 (2018).
C. O'Neil, C. Amarasekara, K. Weerakoon-Ratnayake, B. Groos, Z. Jia, V. Singh, S. Park, S. Soper, Electrokinetic transport properties of deoxynucleotide monophosphates (dNMPs) through thermoplastic nanochannels, Anal. Chim. Acta 1027, 67 (2018).
K. Otomo, T. Hibi, Y.-C. Fang, J.-H. Hung, M. Tsutsumi, R. Kawakami, H. Yokoyama, T. Nemoto, Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts, Biomedical Optics Express 9, 2671 (2018).
F. Ruggeri, M. Krishnan, Entropic Trapping of a Singly Charged Molecule in Solution, Nano letters 18, 3773 (2018).
F. Ruggeri, M. Krishnan, Spectrally resolved single-molecule electrometry, The Journal of Chemical Physics 148, 123307 (2018).
A. Taylor, R. Verhoef, M. Beuwer, Y. Wang, P. Zijlstra, All-Optical Imaging of Gold Nanoparticle Geometry Using Super-Resolution Microscopy, The Journal of Physical Chemistry C 122, 2336 (2018).
W. Chen, L. Young, M. Lu, A. Zaccone, F. Ströhl, N. Yu, G. Kaminski Schierle, C. Kaminski, Fluorescence Self-Quenching from Reporter Dyes Informs on the Structural Properties of Amyloid Clusters Formed in Vitro and in Cells, Nano letters 17, 143 (2017).
A. Murata, Y. Itoh, E. Mano, S. Kanbayashi, C. Igarashi, H. Takahashi, S. Takahashi, K. Kamagata, One-Dimensional Search Dynamics of Tumor Suppressor p53 Regulated by a Disordered C-Terminal Domain, Biophysical Journal 112, 2301 (2017).
D. Nino, N. Rafiei, Y. Wang, A. Zilman, J. Milstein, Molecular Counting with Localization Microscopy, Biophysical Journal 112, 1777 (2017).
F. Ruggeri, F. Zosel, N. Mutter, M. Różycka, M. Wojtas, A. Ożyhar, B. Schuler, M. Krishnan, Single-molecule electrometry, Nature Nanotechnology 12, 488 (2017).
C. Barnaba, M. Martinez, E. Taylor, A. Barden, J. Brozik, Single-Protein Tracking Reveals That NADPH Mediates the Insertion of Cytochrome P450 Reductase into a Biomimetic of the Endoplasmic Reticulum, Journal of the American Chemical Society 139, 5420 (2017).
C. Barnaba, E. Taylor, J. Brozik, Dissociation Constants of Cytochrome P450 2C9/Cytochrome P450 Reductase Complexes in a Lipid Bilayer Membrane Depend on NADPH, Journal of the American Chemical Society 139, 17923 (2017).
R. Hure, S. Simoneau, B. Chandler, J. Steele, Two-Color Metal-Enhanced Fluorescence Using Gold Slotted Nanogratings, Plasmonics 12, 1621 (2017).
E. Jain, S. Sheth, K. Polito, S. Sell, S. Zustiak, Storage stability of biodegradable polyethylene glycol microspheres, Materials Research Express 4, 105403 (2017).
H. Stein, S. Spindler, N. Bonakdar, C. Wang, V. Sandoghdar, Production of Isolated Giant Unilamellar Vesicles under High Salt Concentrations, Frontiers in physiology 8, 63 (2017).
D. Subekti, A. Murata, Y. Itoh, S. Fukuchi, H. Takahashi, S. Kanbayashi, S. Takahashi, K. Kamagata, The Disordered Linker in p53 Participates in Nonspecific Binding to and One-Dimensional Sliding along DNA Revealed by Single-Molecule Fluorescence Measurements, Biochemistry 56, 4134 (2017).
K. Blythe, K. Willets, Super-Resolution Imaging of Fluorophore-Labeled DNA Bound to Gold Nanoparticles, The Journal of Physical Chemistry C 120, 803 (2016).
J. Lee, J. Kim, T.‐C. Yen, I. Lee, B. Ahn, Y. Lee, C.‐L. Hsieh, H. Kim, Y. Jung, A Rhizavidin Monomer with Nearly Multimeric Avidin‐Like Binding Stability Against Biotin Conjugates, Angewandte Chemie International Edition 55, 3393 (2016).
F. Schuler, M. Trotter, R. Zengerle, F. von Stetten, Monochrome Multiplexing in Polymerase Chain Reaction by Photobleaching of Fluorogenic Hydrolysis Probes, Analytical Chemistry 88, 2590 (2016).
S. Sidenstein, E. D'Este, M. Bohm, J. Danzl, V. Belov, S. Hell, Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses, Scientific Reports 6, 26725 (2016).
F. Stockmar, A. Kobitski, G. Nienhaus, Fast Folding Dynamics of an Intermediate State in RNase H Measured by Single-Molecule FRET, The Journal of Physical Chemistry B 120, 641 (2016).
K. Vogele, J. List, G. Pardatscher, N. Holland, F. Simmel, T. Pirzer, Self-Assembled Active Plasmonic Waveguide with a Peptide-Based Thermomechanical Switch, ACS Nano 10, 11377 (2016).
K. Blythe, E. Titus, K. Willets, Effects of Tuning Fluorophore Density, Identity, and Spacing on Reconstructed Images in Super-Resolution Imaging of Fluorophore-Labeled Gold Nanorods, The Journal of Physical Chemistry C 119, 28099 (2015).
G. Cserép, A. Herner, P. Kele, Bioorthogonal fluorescent labels, Methods and Applications in Fluorescence 3, 42001 (2015).
A. Hartmann, G. Krainer, S. Keller, M. Schlierf, Quantification of Millisecond Protein-Folding Dynamics in Membrane-Mimetic Environments by Single-Molecule Forster Resonance Energy Transfer Spectroscopy, Analytical Chemistry 87, 11224 (2015).
J.-W. Janiesch, M. Weiss, G. Kannenberg, J. Hannabuss, T. Surrey, I. Platzman, J. Spatz, Key Factors for Stable Retention of Fluorophores and Labeled Biomolecules in Droplet-Based Microfluidics, Analytical Chemistry 87, 2063 (2015).
E. Kopperger, T. Pirzer, F. Simmel, Diffusive Transport of Molecular Cargo Tethered to a DNA Origami Platform, Nano letters 15, 2693 (2015).
G. Krainer, A. Hartmann, M. Schlierf, farFRET: Extending the Range in Single-Molecule FRET Experiments beyond 10 nm, Nano letters 15, 5826 (2015).
R. Laine, A. Albecka, S. van de Linde, E. Rees, C. Crump, C. Kaminski, Structural analysis of herpes simplex virus by optical super-resolution imaging, Nature Communications 6, 5980 (2015).
M. Lehmann, B. Gottschalk, D. Puchkov, P. Schmieder, S. Schwagerus, C. Hackenberger, V. Haucke, J. Schmoranzer, Multicolor Caged dSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain, Angewandte Chemie International Edition 54, 13230 (2015).
Y. Liu, J. Feshitan, M.-Y. Wei, M. Borden, B. Yuan, Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles, Journal of Biomedical Optics 20, 36012 (2015).
A. Ponomarenko, V. Brylev, E. Nozhevnikova, V. Korshun, Recent Advances in Self-Assembled Fluorescent DNA Structures and Probes, Current topics in medicinal chemistry 15, 1162 (2015).
A. Trapaidze, M. Brut, S. Mazères, D. Estève, A.-M. Gué, A. Bancaud, Thrombin detection in murine plasma using engineered fluorescence resonance energy transfer aptadimers, Applied Physics Letters 107, 233701 (2015).
Y. WU, X. WU, R. Lu, J. Zhang, L. TORO, E. STEFANI, Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy, Scientific Reports 5, 14766 (2015).
M. Aydemir, V. Jankus et al., The key role of geminate electron–hole pair recombination in the delayed fluorescence in rhodamine 6G and ATTO-532, Phys. Chem. Chem. Phys. 16, 21543 (2014).
R. Amor, S. Mahajan et al., Standing-wave-excited multiplanar fluorescence in a laser scanning microscope reveals 3D information on red blood cells, Scientific Reports 4, 7359 (2014).
Y. Liu, J.A. Feshitan et al., Ultrasound-modulated fluorescence based on fluorescent microbubbles, J. Biomed. Opt. 19, 08505 (2014).
R.M.A. Vergauwe, H. Uji-I et al, Shear-Stress-Induced Conformational Changes of von Willebrand Factor in a Water−Glycerol Mixture Observed with Single Molecule Microscopy, J. Phys. Chem. B. 118, 5660 (2014).
M. Davies, B. Rühle et al., Insights into Nanoscale Electrophoresis of Single Dye Molecules in Highly Oriented Mesoporous Silica Channels, J. Phys. Chem. C 118, 24013 (2014).
A. Hartmann, G. Krainer, M. Schlierf, Different Fluorophore Labeling Strategies and Desings Affect Millisecond Kinetics of DNA Hairpins, Molecules 19, 13735 (2014).
J. List, M. Weber, F.C. Simmel, Hydrophobic Actuation of a DNA Origami Bilayer Structure, Angew. Chem. Int. Ed. 53, 4236 (2014).
C.-L. Hsieh, S. Spindler et al., Tracking Single Particles on Supported Lipid Membranes: Multimobility Diffusion and Nanoscopic Confinement, J. Phys. Chem. B 118, 1545 (2014).
M.A. Lauterbach, M. Guillon et al., STED microscope with Spiral Phase Contrast, Scientific Reports 3, article 2050 (2013).
R.C. den Dulk, K.A. Schmidt et al., Magneto-capillary valve for integrated purification and enrichment of nucleic acids and proteins, Lab Chip 13, 106 (2013).
G. Belcastro, C. Mónico et al., Combined single-molecule manipulation and localization for the study of lac Repressor 1D-diffusion along DNA, Proc. SPIE 8810, 88102O (2013).
G. Belcastro, C. Mónico et al., Effect of stretching DNA on lac Repressor 1D-diffusion revealed by the combination of single-molecule localization and manipulation, Proc. SPIE 8797, 87970M (2013).
S.J. Wijeyesakere, S.M. Rizvi, M. Raghavan, Glycan-dependent and -independent Interactions Contribute to Cellular Substrate Recruitment by Calreticulin, J. Biol. Chem. 288, 35104 (2013)
C.-L. Hsieh, S. Spindler et al., Tracking Single Particles on Supported Lipid Membranes: Multimobility Diffusion and Nanoscopic Confinement, J. Phys. Chem. B 118, 1545 (2014).
F. Feil, S. Naumov et al., Single-Particle and Ensemble Diffusivities—Test of Ergodicity, Angew. Chem. 124, 1178 (2012).
K. Kamagata, T. Kawaguchi et al., Long-Term Observation of Fluorescence of Free Single Molecules To Explore Protein-Folding Energy Landscapes, J. Am. Chem. Soc. 134, 11525 (2012).
J. F. Lesione, P. Venkataraman et al., Nanochannel-Based Single Molecule Recycling, Nano Lett. 12, 3273 (2012).
T. Saito, S. Takahashi et al., Platinum plasmonic nanostructure arrays for massively parallel single-molecule detection based on enhanced fluorescence measurements, Nanotechnology 22, 445708 (2011).
F. Feil, S. Naumov et al., Single-Particle and Ensemble Diffusivities—Test of Ergodicity, Angew. Chem. 123, 1 (2011).
D. Aquino, A. Schönle et al., Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores, Nature Methods 8, 353 (2011).
M. Marcinowski, M. Höller et al., Substrate discrimination of the chaperone BiP by autonomous and cochaperone-regulated conformational transitions, NSMB 18, 150 (2011).
R. Bienert, B. Zimmermann et al., Time-Dependent FRET with Single Enzymes: Domain Motions and Catalysis in H+-ATP Synthases, ChemPhysChem 12, 510 (2011).
R. L. Farrow, C. C. Hayden, Molecular-Scale Measurements of Electric Fields at Electrochemical Interfaces, Sandia Reports SAND2011-0172 (2011).
R. Gill, G. W. Lucassen, SERRS-based detection of dye-labeled DNA using positively-charged Ag nanoparticles, Anal. Methods 2, 445 (2010).
G. Donner, C. Eggeling, S.W. Hell, Triplet-relaxation microscopy with bunched pulsed excitation, Photochem. Photobiol. Sci. 8, 451 (2009).
O. Gustafsson, K. B. Mogensen, J.P. Kutter, Underivatized cyclic olefin copolymer as substrate material and stationary phase for capillary and microchip electrochromatography, Electrophoresis 29, 3145 (2008).
A. Punge, S.O. Rizzoli et al., 3D Reconstruction of High-Resolution STED Microsopy Images, Microscopy Res. 71, 644 (2008).
S. Sharma, K. Chakraborty, B. Müller et al., Monitoring Protein Conformation along the Pathway of Chaperonin-Assisted Folding, Cell 133, 142 (2008).
C. Chen, W. Wang, Z. Wang, F. Wie, X.S. Zhao, Influence of secondary structure on kinetics and reaction mechanism of DNA hybridization, Nucl. Acids Res. 35, 2875 (2007).
S. Bretschneider, C. Eggeling, S.W. Hell, Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving, Phys. Rev. Lett. 98, 218103 (2007).
G. Donnert, J. Keller, R. Medda, M.A. Andrei, S.O. Rizzoli et al., Macromolecular-scale resolution in biological fluorescence microscopy, PNAS 103, 11440 (2006).
K.I. Willig, S.O. Rizzoli, V. Westphal et al., STED microscopy reveals that synaptotogamin remains clustered after synaptic vesicle exocytosis, Nature 440, 935 (2006).
K.I. Willig, J. Keller, M. Bossi, S.W. Hell, STED microscopy reveals nanoparticle assemblies, New J. Phys. 8, 1 (2006).
B. Müller, A. Reuter, F. Simmel, D. Lamb, Single-Pair FRET Characterisation of DNA Tweezwers, Nano Lett. 6, 2814 (2006).
A. Armbrüster, C. Hohn, A. Hermesdorf, K. Schumacher, M. Börsch, G. Gruber, Evidence for major structural changes in subunit C of the vacuolar ATPase due to nucleotide binging, FEBS Lett. 759, 1961 (2005).
B. Müller, E. Zaychikov, C. Bräuchle, D. Lamb, Pulsed Interleaved Excitation, Biophys. J. 89, 3508 (2005).
D. Lamb, B. Müller, C. Bräuchle, Enhancing the Sensitivity of Fluorescence Cerrelation Spectroscopy by Using Time-Correlated Single-Photon Counting, Curr. Pharmaceut. Biol. 6, 405 (2005).