ATTO 655
Optische Eigenschaften |
||||
λabs |
663 |
nm |
||
εmax |
1,25×105 |
M-1 cm-1 |
||
λfl |
680 |
nm |
||
ηfl |
30 |
% |
||
τfl |
1,8 |
ns |
||
CF260 = ε260/εmax |
0,24 |
|
||
CF280 = ε280/εmax |
0,08 |
|
ATTO 655 gehört zusammen mit ATTO 680 und ATTO 700 zu einer neuen Generation von langwelligen Fluoreszenzmarkern. Zu den charakteristischen Eigenschaften des Farbstoffs zählen starke Absorption, hohe Fluoreszenzquantenausbeute, hohe thermische und photochemische Stabilität sowie außergewöhnlich hohe Stabilität gegenüber atmosphärischem Ozon. ATTO 655 ist somit besonders geeignet im Bereich der Einzelmoleküldetektion sowie zur hochauflösenden Mikroskopie (PALM, dSTORM, STED, etc.).
Der Farbstoff ist sehr hydrophil und gut wasserlöslich. Nach der Kopplung an ein Substrat ist der Farbstoff elektrisch neutral. ATTO 655 ist ein guter Elektronenakzeptor, seine Fluoreszenz wird durch Guanin, Tryptophan etc. gelöscht. Eine effiziente Anregung der Fluoreszenz erfolgt im Bereich 640 - 675 nm. Für den Farbstoff geeignete Anregungslichtquellen sind der Krypton-Ionen-Laser (647 nm) oder gängige Diodenlaser mit Emission bei 650 nm.
Produktdatenblätter
- ATTO 655 Produktinformation
- NHS-Ester
- Maleimid
- Iodacetamid
- Phalloidin
- Amin
- Azid/Alkin
- Streptavidin
- Tetrazin (MeTet)
Absorptions- und Emissionsspektrum (ASCII)
- ATTO 655 Absorption (.txt)
- ATTO 655 Emission (.txt)
Absorptions- und Emissionsspektrum (Grafik)
- ATTO 655 Absorption/Fluoreszenz (.jpg)
Sicherheitsdatenblätter
- ATTO 655 Carboxy MSDS
- ATTO 655 NHS-Ester MSDS
- ATTO 655 Maleimid MSDS
- ATTO 655 Streptavidin MSDS
- ATTO 655 Biotin MSDS
- ATTO 655 Phalloidin MSDS
- ATTO 655 Amin MSDS
- ATTO 655 Azid MSDS
- ATTO 655 Iodacetamid MSDS
- ATTO 655 Alkin MSDS
- ATTO 655 Cadaverin MSDS
- ATTO 655 MeTet MSDS
Ausgewählte Literatur zu ATTO 655:
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