ATTO 514

ATTO 514

Optical Properties
λ_abs	511	nm
ε_max	1.15×10⁵	M^-1cm^-1
λ_fl	532	nm
η_fl	85	%
τ_fl	3.9	ns
CF₂₆₀ = ε₂₆₀/ε_max	0.21
CF₂₈₀ = ε₂₈₀/ε_max	0.07

ATTO 514 is a new hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield, and exceptional thermal and photo-stability. Thus ATTO 514 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is the 514 nm line of an Argon-Ion laser.

Documents

Product Sheet

- ATTO 514 product information

- additional information
- NHS-ester
- maleimide
- iodoacetamide
- phalloidin
- amine
- azide/alkyne
- streptavidin

Spectra

Absorption and Emission Spectrum (ASCII)

- ATTO 514 absorption (.txt)

- ATTO 514 emission (.txt)

Absorption and Emission Spectrum (grafic)

- ATTO 514 absorption/fluorescence (.jpg)

MSDS

Material Safety Data Sheets

- ATTO 514 Carboxy MSDS

- ATTO 514 NHS-ester MSDS

- ATTO 514 maleimide MSDS

- ATTO 514 streptavidin MSDS

- ATTO 514 biotin MSDS

- ATTO 514 phalloidin MSDS

- ATTO 514 amine MSDS

- ATTO 514 azide MSDS

- ATTO 514 iodoacteamide MSDS

- ATTO 514 hydrazide MSDS

- ATTO 514 alkyne MSDS

Literature

Selected Publications for ATTO 514:

J. Seo, Y. Sim, J. Kim, H. Kim, I. Cho, H. Nam, Y.-G. Yoon, J.-B. Chang, PICASSO allows ultra-multiplexed fluorescence imaging of spatially overlapping proteins without reference spectra measurements, Nature Communications 13, 1 (2022).

M. Arizono, V. Inavalli, S. Bancelin, M. Fernández‐Monreal, U. Nägerl, Super‐resolution shadow imaging reveals local remodeling of astrocytic microstructures and brain extracellular space after osmotic challenge, Glia (2021).

A. Yadav, C. Rao, C. Nandi, Fluorescent Probes for Super-Resolution Microscopy of Lysosomes, ACS Omega 5, 26967 (2020).

H.-Y. Hsieh, Y.-H. Peng, S.-F. Lin, L.-C. Chen, T.-C. Yu, C.-F. Chiou, J. Lee, Triple-Junction Optoelectronic Sensor with Nanophotonic Layer Integration for Single-Molecule Level Decoding, ACS Nano 13, 4486 (2019).

M. Zhang, M. Li, W. Zhang, Y. Han, Y.-H. Zhang, Simple and efficient delivery of cell-impermeable organic fluorescent probes into live cells for live-cell superresolution imaging, Light: Science & Applications 8, 1 (2019).

D. Beer, M. Botes, T. Cloete, The microbial community of a biofilm contact reactor for the treatment of winery wastewater, Journal of Applied Microbiology 124, 598 (2018).

D. Hammler, A. Marx, A. Zumbusch, Fluorescence-Lifetime-Sensitive Probes for Monitoring ATP Cleavage, Chemistry (Weinheim an der Bergstrasse, Germany) 24, 15329 (2018).

J. Tønnesen, V. Inavalli, U. Nägerl, Super-Resolution Imaging of the Extracellular Space in Living Brain Tissue, Cell 172, 1108-1121.e15 (2018).

M. Lehmann, B. Gottschalk, D. Puchkov, P. Schmieder, S. Schwagerus, C. Hackenberger, V. Haucke, J. Schmoranzer, Multicolor Caged dSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain, Angewandte Chemie International Edition 54, 13230 (2015).

Product No.:: AD 514

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ATTO 514

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