ATTO 532
Optical Properties |
||||
λabs |
532 |
nm |
||
εmax |
1.15×105 |
M-1 cm-1 |
||
λfl |
552 |
nm |
||
ηfl |
90 |
% |
||
τfl |
3.8 |
ns |
||
CF260 = ε260/εmax |
0.20 |
|
||
CF280 = ε280/εmax |
0.09 |
|
ATTO 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photo-stability, and excellent water solubility. Thus ATTO 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 515 - 545 nm. A suitable excitation source for ATTO 532 is the 532 nm output of the frequency-doubled Nd:YAG laser.
Product Sheets
- ATTO 532 product information
- NHS-ester
- maleimide
- iodoacetamide
- phalloidin
- amine
- azide/alkyne
- streptavidin
- tetrazine (MeTet)
Absorption and Emission Spectrum (ASCII)
- ATTO 532 absorption (.txt)
- ATTO 532 emission (.txt)
Absorption and Emission Spectrum (graphic)
- ATTO 532 absorption/fluorescence (.jpg)
Material Safety Data Sheets
- ATTO 532 carboxy MSDS
- ATTO 532 NHS-ester MSDS
- ATTO 532 maleimide MSDS
- ATTO 532 streptavidin MSDS
- ATTO 532 biotin MSDS
- ATTO 532 phalloidin MSDS
- ATTO 532 amine MSDS
- ATTO 532 azide MSDS
- ATTO 532 iodoacetamide MSDS
- ATTO 532 hydrazide MSDS
- ATTO 532 alkyne MSDS
- ATTO 532 cadaverine MSDS
- ATTO 532 MeTet MSDS
- ATTO 532 Peg(4)-DBCO MSDS
Selscted Publications for ATTO 532:
G. Campagnola, O. Peersen, Co-folding and RNA activation of poliovirus 3Cpro polyprotein precursors, Journal of Biological Chemistry 299, 105258 (2023).
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M. Schenkel, D. Ravamehr-Lake, T. Czerniak, J. Saenz, G. Krainer, M. Schlierf, C. Deber, Impact of cholesterol and Lumacaftor on the folding of CFTR helical hairpins, Biochimica et Biophysica Acta (BBA) - Biomembranes 1865, 184078 (2023).
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