ATTO 550
Optical Properties |
||||
λabs |
554 |
nm |
||
εmax |
1.2×105 |
M-1 cm-1 |
||
λfl |
576 |
nm |
||
ηfl |
80 |
% |
||
τfl |
3.6 |
ns |
||
CF260 = ε260/εmax |
0.23 |
|
||
CF280 = ε280/εmax |
0.10 |
|
ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO 550 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. As supplied ATTO 550 consists of three isomers with practically identical absorption and fluorescence. The fluorescence is excited most efficiently in the range 540 - 565 nm.
Product Sheets
- ATTO 550 product information
- NHS-ester
- maleimide
- iodoacetamide
- phalloidin
- amine
- azide/alkyne
- streptavidin
- tetrazine (MeTet)
Absorption and Emission Spectrum (ASCII)
- ATTO 550 absorption (.txt)
- ATTO 550 emission (.txt)
Absorption and Emission Spectrum (graphic)
- ATTO 550 absorption/emission (.jpg)
Material Safety Data Sheets
- ATTO 550 carboxy MSDS
- ATTO 550 NHS-ester MSDS
- ATTO 550 maleimide MSDS
- ATTO 550 streptavidin MSDS
- ATTO 550 biotin MSDS
- ATTO 550 phalloidin MSDS
- ATTO 550 amine MSDS
- ATTO 550 azide MSDS
- ATTO 550 iodoacetamide MSDS
- ATTO 550 hydrazide MSDS
- ATTO 550 alkyne MSDS
- ATTO 550 cadaverine MSDS
- ATTO 550 MeTet MSDS
- ATTO 550 Peg(4)-DBCO MSDS
Selected Publications for ATTO 550:
Z. Baka, C. Godier, L. Lamy, A. Mallick, V. Gribova, A. Figarol, L. Bezdetnaya, A. Chateau, Z. Magne, M. Stiefel, D. Louaguef, P. Lavalle, E. Gaffet, O. Joubert, H. Alem, A Coculture Based, 3D Bioprinted Ovarian Tumor Model Combining Cancer Cells and Cancer Associated Fibroblasts, Macromolecular Bioscience 23, 2200434 (2023).
P. Bourceau, B. Geier, V. Suerdieck, T. Bien, J. Soltwisch, K. Dreisewerd, M. Liebeke, Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling, Nature Protocols 18, 3050 (2023).
V. Dolfo, E. Boissin, M. Tatarata, S. Planes, Characterization of 25 new microsatellite markers for the green turtle (Chelonia mydas) and cross-species amplification in other marine turtle species, Molecular biology reports 50, 4145 (2023).
M. Estévez, M. Cicuéndez, J. Crespo, J. Serrano-López, M. Colilla, C. Fernández-Acevedo, T. Oroz-Mateo, A. Rada-Leza, B. González, I. Izquierdo-Barba, M. Vallet-Regí, Large-scale production of superparamagnetic iron oxide nanoparticles by flame spray pyrolysis, Journal of Colloid and Interface Science 650, 560 (2023).
G. Filoglu, A. Duvenci, S. Tas, H. Karadayi, F. Asicioglu, O. Bulbul, Development of a multiplex panel with 36 insertion/deletion markers (InDel) for individual identification, Forensic science, medicine, and pathology (2023).
J. Hwang, K. Kim, S. Seo, Y. Nam, S. Jwa, I. Yang, T. Park, O. Kwon, S. Lee, Bioelectronic Tongue for Identifying and Masking Bitterness Based on Bitter Taste Receptor Agonism and Antagonism, Environmental Toxicology 33, 2304997 (2023).
C. Leoni, M. Bataclan, T. Ito-Kureha, V. Heissmeyer, S. Monticelli, The mRNA methyltransferase Mettl3 modulates cytokine mRNA stability and limits functional responses in mast cells, Nature communications 14, 3862 (2023).
N. Ponzar, N. Pozzi, Probing the conformational dynamics of thiol-isomerases using non-canonical amino acids and single-molecule FRET, Methods 214, 8 (2023).
S. Pradhan, S. Apaydin, J. Bucevičius, R. Gerasimaitė, G. Kostiuk, G. Lukinavičius, Sequence-specific DNA labelling for fluorescence microscopy, Biosensors and Bioelectronics 230, 115256 (2023).
E. Schmidt, D. Lamprinaki, K. McCord, M. Joe, M. Sojitra, A. Waldow, J. Nguyen, J. Monyror, E. Kitova, F. Mozaneh, X. Guo, J. Jung, J. Enterina, G. Daskhan, L. Han, A. Krysler, C. Cromwell, B. Hubbard, L. West, M. Kulka, S. Sipione, J. Klassen, R. Derda, T. Lowary, L. Mahal, M. Riddell, M. Macauley, Siglec-6 mediates the uptake of extracellular vesicles through a noncanonical glycolipid binding pocket, Nature communications 14, 2327 (2023).
S. Soeda, D. Ito, T. Ogushi, Y. Sano, R. Negoro, T. Fujita, R. Saito, H. Taniura, Defects in early synaptic formation and neuronal function in Prader-Willi syndrome, Scientific reports 13, 12053 (2023).
V. Vargová, D. Gužiová, M. Balogová, N. Pipová, M. Uhrin, P. Kaňuch, Urban environment determines population genetics in the green toad, Bufotes viridis, European Journal of Wildlife Research 69, 384 (2023).
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M. Chinnaraj, R. Flaumenhaft, N. Pozzi, Reduction of protein disulfide isomerase results in open conformations and stimulates dynamic exchange between structural ensembles, Journal of Biological Chemistry 298, 102217 (2022).
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L. Cruz, T. van Dijk, O. Vepris, T. Li, T. Schomann, F. Baldazzi, R. Kurita, Y. Nakamura, F. Grosveld, S. Philipsen, C. Eich, PLGA-Nanoparticles for Intracellular Delivery of the CRISPR-Complex to Elevate Fetal Globin Expression in Erythroid Cells, Biomaterials 268, 120580 (2021).
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J. Reyes, S. Ekmark-Léwen, M. Perdiki, T. Klingstedt, A. Hoffmann, E. Wiechec, P. Nilsson, K. Nilsson, I. Alafuzoff, M. Ingelsson, M. Hallbeck, Accumulation of alpha-synuclein within the liver, potential role in the clearance of brain pathology associated with Parkinson's disease, Acta neuropathologica communications 9, 46 (2021).
R.-L. Troskie, Y. Jafrani, T. Mercer, A. Ewing, G. Faulkner, S. Cheetham, Long-read cDNA sequencing identifies functional pseudogenes in the human transcriptome, Genome Biology 22, 1 (2021).
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J. Wardyn, A. Chan, A. Jeyasekharan, A Robust Protocol for CRISPR-Cas9 Gene Editing in Human Suspension Cell Lines, Current Protocols 1, e286 (2021).
S. Yeou, N. Lee, Contribution of a DNA Nick to DNA Bendability Depending on the Bending Force, Bulletin of the Korean Chemical Society 42, 1151 (2021).
M. Zoppo, N. Okoniewski, S. Pantelyushin, J. Vom Berg, K. Schirmer, A ribonucleoprotein transfection strategy for CRISPR/Cas9‐mediated gene editing and single cell cloning in rainbow trout cells, Cell & Bioscience 11, 1 (2021).
A. Al Masud, W. Martin, F. Moonschi, S. Park, B. Srijanto, K. Graham, C. Collier, C. Richards, Mixed metal zero-mode guides (ZMWs) for tunable fluorescence enhancement, Nanoscale Advances 2, 1894 (2020).
M. Barbiero, S. Castelletto, Q. Zhang, Y. Chen, M. Charnley, S. Russell, M. Gu, Nanoscale magnetic imaging enabled by nitrogen vacancy centres in nanodiamonds labelled by iron-oxide nanoparticles, Nanoscale 12, 8847 (2020).
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A.-K. Schneider, T. Scharnweber, D. Cammann, B. Rapp, S. Giselbrecht, C. Niemeyer, Multiscale Microstructure for Investigation of Cell–Cell Communication, Small Methods 4, 2000647 (2020).
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J. Spitzberg, X. van Kooten, M. Bercovici, A. Meller, Microfluidic device for coupling isotachophoretic sample focusing with nanopore single-molecule sensing, Nanoscale 12, 17805 (2020).
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K. Banas, N. Rivera-Torres, P. Bialk, B.-C. Yoo, E. Kmiec, Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells, Molecular cancer research : MCR 18, 891 (2020).
N. Gilat, D. Torchinsky, S. Margalit, Y. Michaeli, S. Avraham, H. Sharim, N. Elkoshi, C. Levy, S. Zirkin, Y. Ebenstein, Rapid Quantification of Oxidation and UV Induced DNA Damage by Repair Assisted Damage Detection-(Rapid RADD), Analytical Chemistry 92, 9887 (2020).
K. Göpfrich, M. Urban, C. Frey, I. Platzman, J. Spatz, N. Liu, Dynamic Actuation of DNA-Assembled Plasmonic Nanostructures in Microfluidic Cell-Sized Compartments, Nano letters 20, 1571 (2020).
D. Hastman, J. Melinger, G. Aragonés, P. Cunningham, M. Chiriboga, Z. Salvato, T. Salvato, C. Brown, D. Mathur, I. Medintz, E. Oh, S. Díaz, Femtosecond Laser Pulse Excitation of DNA-Labeled Gold Nanoparticles, ACS Nano (2020).
S. Patra, M. Baibakov, J.-B. Claude, J. Wenger, Surface passivation of zero-mode waveguide nanostructures, Scientific Reports 10, 1 (2020).
Á. Molinero-Fernández, M. Moreno-Guzmán, L. Arruza, M. López, A. Escarpa, Polymer-Based Micromotor Fluorescence Immunoassay for On-the-Move Sensitive Procalcitonin Determination in Very Low Birth Weight Infants’ Plasma, ACS Sensors 5, 1336 (2020).
R. Ankri, A. Basu, A. Ulku, C. Bruschini, E. Charbon, S. Weiss, X. Michalet, Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium, ACS Photonics 7, 68 (2019).
M. Baibakov, S. Patra, J.-B. Claude, A. Moreau, J. Lumeau, J. Wenger, Extending Single-Molecule Förster Resonance Energy Transfer (FRET) Range beyond 10 Nanometers in Zero-Mode Waveguides, ACS Nano 13, 8469 (2019).
S. Chavan, D. Kim, J. Hwang, Y. Choi, J. Hong, J. Kim, M.-H. Lee, M. Hwang, J. Choi, Enhanced Detection of Infectious Pancreatic Necrosis Virus via Lateral Flow Chip and Fluorometric Biosensors Based on Self-Assembled Protein Nanoprobes, ACS Sensors 4, 2937 (2019).
B. Dalzon, A. Torres, H. Diemer, S. Ravanel, V. Collin-Faure, K. Pernet-Gallay, P.-H. Jouneau, J. Bourguignon, S. Cianférani, M. Carrière, C. Aude-Garcia, T. Rabilloud, How reversible are the effects of silver nanoparticles on macrophages?, Environmental Science: Nano 6, 3133 (2019).
I. Hoffecker, S. Chen, A. Gådin, A. Bosco, A. Teixeira, B. Högberg, Solution‐Controlled Conformational Switching of an Anchored Wireframe DNA Nanostructure, Small 15 (2019).
C. Kimna, O. Lieleg, Engineering an orchestrated release avalanche from hydrogels using DNA-nanotechnology, Journal of Controlled Release 304, 19 (2019).
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Y. Li, A. Chukun Li, Q. Xu, Intracellular Delivery of His‐Tagged Genome‐Editing Proteins Enabled by Nitrilotriacetic Acid–Containing Lipidoid Nanoparticles, Advanced Healthcare Materials 8 (2019).
G. Lin, M. Lewandowska, Plasmon-enhanced fluorescence provided by silver nanoprisms for sensitive detection of sulfide, Sensors and Actuators B: Chemical 292, 241 (2019).
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M. Sauer, S. Juranek, J. Marks, A. de Magis, H. Kazemier, D. Hilbig, D. Benhalevy, X. Wang, M. Hafner, K. Paeschke, DHX36 prevents the accumulation of translationally inactive mRNAs with G4-structures in untranslated regions, Nature Communications 10, 2421 (2019).
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