ATTO 565
Optical Properties |
||||
λabs |
564 |
nm |
||
εmax |
1.2×105 |
M-1 cm-1 |
||
λfl |
590 |
nm |
||
ηfl |
90 |
% |
||
τfl |
4.0 |
ns |
||
CF260 = ε260/εmax |
0.27 |
|
||
CF280 = ε280/εmax |
0.12 |
|
ATTO 565 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 565 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.
The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 545 - 575 nm. As supplied ATTO 565 consists of a mixture of two isomers with practically identical absorption and fluorescence. However, on request the dye can also be supplied as single isomer.
Product Sheet
- ATTO 565 product information
- additional information
- NHS-ester
- maleimide
- iodoacetamide
- phalloidin
- amine
- azide/alkyne
- streptavidin
Absorption and Emission Spectrum (ASCII)
- ATTO 565 absorption (.txt)
- ATTO 565 emission (.txt)
Absorption and Emission Spectrum (graphic)
- ATTO 565 absorption/fluorescence (.jpg)
Material Safety Data Sheets
- ATTO 565 carboxy MSDS
- ATTO 565 NHS-ester MSDS
- ATTO 565 maleimide MSDS
- ATTO 565 streptavidin MSDS
- ATTO 565 biotin MSDS
- ATTO 565 phalloidin MSDS
- ATTO 565 amine MSDS
- ATTO 565 azide MSDS
- ATTO 565 iodoacetamide MSDS
- ATTO 565 hydrazide MSDS
- ATTO 565 alkyne MSDS
- ATTO 565 cadaverine MSDS
- ATTO 565 tetrazine (MeTet) MSDS
- ATTO 565 Peg(4)-DBCO MSDS
Selected Publications for ATTO 565:
J. Aretz, M. Aziz, N. Strohmeyer, M. Sattler, R. Fässler, Talin and kindlin use integrin tail allostery and direct binding to activate integrins, Nature structural & molecular biology 30, 1913 (2023).
L. Bruurs, M. Müller, J. Schipper, H. Rabouw, S. Boersma, F. van Kuppeveld, M. Tanenbaum, Antiviral responses are shaped by heterogeneity in viral replication dynamics, Nature microbiology 8, 2115 (2023).
P. Dave, G. Roth, E. Griesbach, D. Mateju, T. Hochstoeger, J. Chao, Single-molecule imaging reveals translation-dependent destabilization of mRNAs, Molecular Cell 83, 589-606.e6 (2023).
V. Dolfo, E. Boissin, M. Tatarata, S. Planes, Characterization of 25 new microsatellite markers for the green turtle (Chelonia mydas) and cross-species amplification in other marine turtle species, Molecular biology reports 50, 4145 (2023).
Y. Duan, F. Szlam, Y. Hu, W. Chen, R. Li, Y. Ke, R. Sniecinski, K. Salaita, Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand, Nature biomedical engineering 7, 1404 (2023).
G. Filoglu, A. Duvenci, S. Tas, H. Karadayi, F. Asicioglu, O. Bulbul, Development of a multiplex panel with 36 insertion/deletion markers (InDel) for individual identification, Forensic science, medicine, and pathology (2023).
T. Guitton-Spassky, F. Junge, A. Singh, B. Schade, K. Achazi, M. Maglione, S. Sigrist, R. Rashmi, R. Haag, Fluorinated dendritic amphiphiles, their stomatosome aggregates and application in enzyme encapsulation, Nanoscale 15, 7781 (2023).
M. Hisham, A. Salih, H. Butt, 3D Printing of Multimaterial Contact Lenses, ACS Biomaterials Science & Engineering 9, 4381 (2023).
S. Hittini, A. Salih, F. Alam, A. Shanti, S. Lee, K. Polychronopoulou, H. AlSafar, F. Almaskari, H. Butt, Fabrication of 3D-Printed Contact Lenses and Their Potential as Color Blindness Ocular Aids, Macromolecular Materials and Engineering 308, 2200601 (2023).
C. Jiménez-Jiménez, A. Moreno-Borrallo, B. Dumontel, M. Manzano, M. Vallet-Regí, Biomimetic camouflaged nanoparticles with selective cellular internalization and migration competences, Acta Biomaterialia 157, 395 (2023).
A. Kaufmann, M. Vigogne, T. Neuendorf, M. Reverte-López, G. Rivas, J. Thiele, Studying Nucleoid-Associated Protein–DNA Interactions Using Polymer Microgels as Synthetic Mimics, ACS Synthetic Biology 12, 3695 (2023).
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S. Laber, S. Strobel, J. Mercader, H. Dashti, F. dos Santos, P. Kubitz, M. Jackson, A. Ainbinder, J. Honecker, S. Agrawal, G. Garborcauskas, D. Stirling, A. Leong, K. Figueroa, N. Sinnott-Armstrong, M. Kost-Alimova, G. Deodato, A. Harney, G. Way, A. Saadat, S. Harken, S. Reibe-Pal, H. Ebert, Y. Zhang, V. Calabuig-Navarro, E. McGonagle, A. Stefek, J. Dupuis, B. Cimini, H. Hauner, M. Udler, A. Carpenter, J. Florez, C. Lindgren, S. Jacobs, M. Claussnitzer, Discovering cellular programs of intrinsic and extrinsic drivers of metabolic traits using LipocyteProfiler, Cell Genomics 3, 100346 (2023).
M. Luo, Y. Li, B. Peng, J. White, E. Mäkilä, W. Tong, C. Jonathan Choi, B. Day, N. Voelcker, A Multifunctional Porous Silicon Nanocarrier for Glioblastoma Treatment, Molecular pharmaceutics 20, 545 (2023).
M. Romeiro Motta, S. Biswas, L. Schaedel, Beyond uniformity, European Journal of Cell Biology 102, 151370 (2023).
A. Salih, F. Alam, M. Elsherif, S. Hittini, H. Butt, Low-Cost Customized Color-Blind Contact Lenses using 3D Printed Molds, Advanced Engineering Materials 25, 2300004 (2023).
A. Savulescu, N. Peton, D. Oosthuizen, R. Hazra, R. Rousseau, M. Mhlanga, A. Coussens, Quantifying spatial dynamics of Mycobacterium tuberculosis infection of human macrophages using microfabricated patterns, Cell Reports Methods 3, 100640 (2023).
R. Schmid, M. Volcic, S. Fischer, Z. Qu, H. Barth, A. Popat, F. Kirchhoff, M. Lindén, Surface functionalization affects the retention and bio-distribution of orally administered mesoporous silica nanoparticles in a colitis mouse model, Scientific reports 13, 20175 (2023).
R. Shukla, A. Peoples, K. Ludwig, S. Maity, M. Derks, S. de Benedetti, A. Krueger, B. Vermeulen, T. Harbig, F. Lavore, R. Kumar, R. Honorato, F. Grein, K. Nieselt, Y. Liu, A. Bonvin, M. Baldus, U. Kubitscheck, E. Breukink, C. Achorn, A. Nitti, C. Schwalen, A. Spoering, L. Ling, D. Hughes, M. Lelli, W. Roos, K. Lewis, T. Schneider, M. Weingarth, An antibiotic from an uncultured bacterium binds to an immutable target, Cell 186, 4059-4073.e27 (2023).
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M. Luo, Y. Li, B. Peng, J. White, E. Mäkilä, W. Tong, C. Jonathan Choi, B. Day, N. Voelcker, A Multifunctional Porous Silicon Nanocarrier for Glioblastoma Treatment, Molecular pharmaceutics 20, 545 (2023).
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J. Zalieckas, I. Mondragon, P. Pobedinskas, A. Kristoffersen, S. Mohamed-Ahmed, C. Gjerde, P. Høl, G. Hallan, O. Furnes, M. Cimpan, K. Haenen, B. Holst, M. Greve, Polycrystalline Diamond Coating on Orthopedic Implants, ACS applied materials & interfaces 14, 44933 (2022).
A. de Zélicourt, A. Fayssoil, M. Dakouane-Giudicelli, I. de Jesus, A. Karoui, F. Zarrouki, F. Lefebvre, A. Mansart, J.-M. Launay, J. Piquereau, M. Tarragó, M. Bonay, A. Forand, S. Moog, F. Piétri-Rouxel, E. Brisebard, C. Chini, S. Kashyap, M. Fogarty, G. Sieck, M. Mericskay, E. Chini, A. Gomez, J.-M. Cancela, S. de La Porte, CD38-NADase is a new major contributor to Duchenne muscular dystrophic phenotype, EMBO Molecular Medicine 14, e12860 (2022).
B. Beitzinger, F. Gerbl, T. Vomhof, R. Schmid, R. Noschka, A. Rodriguez, S. Wiese, G. Weidinger, L. Ständker, P. Walther, J. Michaelis, M. Lindén, S. Stenger, Delivery by Dendritic Mesoporous Silica Nanoparticles Enhances the Antimicrobial Activity of a Napsin-Derived Peptide Against Intracellular Mycobacterium tuberculosis, Advanced Healthcare Materials 10, 2100453 (2021).
B. Benham-Pyle, C. Brewster, A. Kent, F. Mann, S. Chen, A. Scott, A. Box, A. Sánchez Alvarado, Identification of rare, transient post-mitotic cell states that are induced by injury and required for whole-body regeneration in Schmidtea mediterranea, Nature Cell Biology 23, 939 (2021).
S. Chakraborty, V. Gourain, M. Benz, J. Scheiger, P. Levkin, A. Popova, Droplet microarrays for cell culture, Materials Today Bio 11, 100112 (2021).
Y. Cheng, M. Liu, M. Hu, S. Wang, TAD-like single-cell domain structures exist on both active and inactive X chromosomes and persist under epigenetic perturbations, Genome Biology 22, 1 (2021).
A. Chiki, J. Ricci, R. Hegde, L. Abriata, A. Reif, D. Boudeffa, H. Lashuel, Site-Specific Phosphorylation of Huntingtin Exon 1 Recombinant Proteins Enabled by the Discovery of Novel Kinases, Encyclopedia of Analytical Chemistry 22, 217 (2021).
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J. Lu, A. Doyle, Y. Shinsato, S. Wang, M. Bodendorfer, M. Zheng, K. Yamada, Basement Membrane Regulates Fibronectin Organization Using Sliding Focal Adhesions Driven by a Contractile Winch, Developmental Cell 52, 631-646.e4 (2020).
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K. Min, I. Cho, M. Choi, J.-B. Chang, Multiplexed expansion microscopy of the brain through fluorophore screening, Methods 174, 3 (2020).
V. Moreno, E. Álvarez, I. Izquierdo-Barba, A. Baeza, J. Serrano-López, M. Vallet-Regí, Bacteria as Nanoparticles Carrier for Enhancing Penetration in a Tumoral Matrix Model, Advanced Materials Interfaces 7, 1901942 (2020).
N. Overeem, P. Hamming, J. Huskens, Time-Dependent Binding of Molecules and Nanoparticles at Receptor-Modified Supported Lipid Bilayer Gradients in a Microfluidic Device, Encyclopedia of Analytical Chemistry 5, 9799 (2020).
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A. Barth, L. Voith von Voithenberg, D. Lamb, Quantitative Single-Molecule Three-Color Förster Resonance Energy Transfer by Photon Distribution Analysis, The journal of physical chemistry. B 123, 6901 (2019).
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Y. Wu, H. Ruan, R. Zhao, Z. Dong, W. Li, X. Tang, J. Yuan, X. Fang, Ultrastable Fluorescent Polymer Dots for Stimulated Emission Depletion Bioimaging, Advanced Optical Materials 6 (2018).
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