ATTO 643

ATTO 643

Optical Properties
λ_abs	643	nm
ε_max	1.5×10⁵	M^-1cm^-1
λ_fl	665	nm
η_fl	62	%
τ_fl	3.5	ns
CF₂₆₀ = ε₂₆₀/ε_max	0.05
CF₂₈₀ = ε₂₈₀/ε_max	0.04

ATTO 643 is a new fluorescent label related to the well known ATTO 647N. Characteristic features of the label are strong absorption and high thermal and photo-stability. The dye is very hydrophilic, hence it shows excellent water solubility and significantly reduced tendency for unspecific binding. ATTO 643 exhibits an extraordinary high fluorescence quantum yield. Contrary to many common fluorescent labels this is the case even when the dye is coupled to proteins, e.g. antibodies, streptavidin etc. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. The label is also highly recommended for oligonucleotide labeling, flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.
In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 643 is an anionic dye. After coupling to a substrate the dye carries a net electrical charge of -1. As supplied ATTO 643 consists of a mixture of three isomers with practically identical absorption and fluorescence properties. The fluorescence is excited most efficiently in the range 625 - 660 nm.

Documents

Product Sheets

- ATTO 643 product information

- NHS-ester
- maleimide
- phalloidin
- amine
- azide/alkyne
- streptavidin

Spectra

Absorption and Emission Spectrum (ASCII)

- ATTO 643 absorption (.txt)

- ATTO 643 emission (.txt)

Absorption and Emission Spectrum (graphic)

- ATTO 643 absorption/fluorescence (.jpg)

MSDS

Material Safety Data Sheets

- ATTO 643 carboxy MSDS

- ATTO 643 NHS-ester MSDS

- ATTO 643 maleimide MSDS

- ATTO 643 streptavidin MSDS

- ATTO 643 biotin MSDS

- ATTO 643 phalloidin MSDS

- ATTO 643 amine MSDS

- ATTO 643 azide MSDS

- ATTO 643 alkyne MSDS

- ATTO 643 Peg(4)-DBCO MSDS

Literature

Selected Publications for ATTO 643:

D. Bessa-Neto, D. Choquet, Molecular mechanisms of AMPAR reversible stabilization at synapses, Molecular and Cellular Neuroscience 125, 103856 (2023).

A. Luginina, I. Maslov, P. Khorn, O. Volkov, A. Khnykin, P. Kuzmichev, M. Shevtsov, A. Belousov, I. Kapranov, D. Dashevskii, D. Kornilov, E. Bestsennaia, J. Hofkens, J. Hendrix, T. Gensch, V. Cherezov, V. Ivanovich, A. Mishin, V. Borshchevskiy, Functional GPCR Expression in Eukaryotic LEXSY System, Journal of Molecular Biology 435, 168310 (2023).

I. Maslov, O. Volkov, P. Khorn, P. Orekhov, A. Gusach, P. Kuzmichev, A. Gerasimov, A. Luginina, Q. Coucke, A. Bogorodskiy, V. Gordeliy, S. Wanninger, A. Barth, A. Mishin, J. Hofkens, V. Cherezov, T. Gensch, J. Hendrix, V. Borshchevskiy, Sub-millisecond conformational dynamics of the A2A adenosine receptor revealed by single-molecule FRET, Communications biology 6, 362 (2023).

H. Maus, G. Hinze, S. Hammerschmidt, T. Basché, T. Schirmeister, A competition smFRET assay to study ligand-induced conformational changes of the dengue virus protease, Protein Science 32, e4526 (2023).

C. Niederauer, C. Nguyen, M. Wang-Henderson, J. Stein, S. Strauss, A. Cumberworth, F. Stehr, R. Jungmann, P. Schwille, K. Ganzinger, Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions, Nature communications 14, 4345 (2023).

S. Tiwari, P. Roy, J.-B. Claude, J. Wenger, Achieving High Temporal Resolution in Single-Molecule Fluorescence Techniques Using Plasmonic Nanoantennas, Small Methods 11, 2300168 (2023).

K. Chung, Z. Zhang, P. Kidd, Y. Zhang, N. Williams, B. Rollins, Y. Yang, C. Lin, D. Baddeley, J. Bewersdorf, Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging, Nature Methods 19, 554 (2022).

M. Kamel, M. Löwe, S. Schott-Verdugo, H. Gohlke, A. Kedrov, Unsaturated fatty acids augment protein transport via the SecA:SecYEG translocon, The FEBS Journal 289, 140 (2022).

A. Koester, M. Szczepaniak, X. Nan, Fast and Multiplexed Super Resolution Imaging of Fixed and Immunostained Cells with DNA-PAINT-ERS, Current Protocols 2, e618 (2022).

M. Lycas, A. Ejdrup, A. Sørensen, N. Haahr, S. Jørgensen, D. Guthrie, J. Støier, C. Werner, A. Newman, M. Sauer, F. Herborg, U. Gether, Nanoscopic dopamine transporter distribution and conformation are inversely regulated by excitatory drive and D2 autoreceptor activity, Cell Reports 40, 111431 (2022).

N. Oleksiievets, C. Mathew, J. Thiele, J. Gallea, O. Nevskyi, I. Gregor, A. Weber, R. Tsukanov, J. Enderlein, Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy, Nano letters 22, 6454 (2022).

J. Sotolongo Bellón, O. Birkholz, C. Richter, F. Eull, H. Kenneweg, S. Wilmes, U. Rothbauer, C. You, M. Walter, R. Kurre, J. Piehler, Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane, Cell Reports Methods 2, 100165 (2022).

T. Trinh, I. Thompson, F. Clark, J. Remington, M. Eisenstein, J. Li, H. Soh, A Photoresponsive Intramolecular Triplex Motif That Enables Rapid and Reversible Control of Aptamer Binding Activity, ACS Nano 16, 14549 (2022).

D. Bessa-Neto, G. Beliu, A. Kuhlemann, V. Pecoraro, S. Doose, N. Retailleau, N. Chevrier, D. Perrais, M. Sauer, D. Choquet, Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons, Nature communications 12, 6715 (2021).

A. Garitano-Trojaola, A. Sancho, R. Götz, P. Eiring, S. Walz, H. Jetani, J. Gil-Pulido, M. Da Via, E. Teufel, N. Rhodes, L. Haertle, E. Arellano-Viera, R. Tibes, A. Rosenwald, L. Rasche, M. Hudecek, M. Sauer, J. Groll, H. Einsele, S. Kraus, M. Kortüm, Actin cytoskeleton deregulation confers midostaurin resistance in FLT3-mutant acute myeloid leukemia, Communications Biology 4, 1 (2021).

M. Kloos, A. Sharma, J. Enderlein, U. Diederichsen, Transmembrane β-peptide helices as molecular rulers at the membrane surface, Journal of Peptide Science 27, e3355 (2021).

A. Yeşilyurt, J.-S. Huang, Emission Manipulation by DNA Origami-Assisted Plasmonic Nanoantennas, Advanced Optical Materials 9, 2100848 (2021).

F. Zheng, M. Kelly, D. Ramms, M. Heintschel, K. Tao, B. Tutuncuoglu, J. Lee, K. Ono, H. Foussard, M. Chen, K. Herrington, E. Silva, S. Liu, J. Chen, C. Churas, N. Wilson, A. Kratz, R. Pillich, D. Patel, J. Park, B. Kuenzi, M. Yu, K. Licon, D. Pratt, J. Kreisberg, M. Kim, D. Swaney, X. Nan, S. Fraley, J. Gutkind, N. Krogan, T. Ideker, Interpretation of cancer mutations using a multiscale map of protein systems, Science 374, 2825 (2021).

M. Alghamdi, F. Chierchini, D. Eigel, C. Taplan, T. Miles, D. Pette, P. Welzel, C. Werner, W. Wang, C. Neto, M. Gumbleton, B. Newland, Poly(ethylene glycol) based nanotubes for tuneable drug delivery to glioblastoma multiforme, Nanoscale Advances 2, 4498 (2020).

C. Gorby, J. Sotolongo Bellón, S. Wilmes, W. Warda, E. Pohler, P. Fyfe, A. Cozzani, C. Ferrand, M. Walter, S. Mitra, J. Piehler, I. Moraga, Engineered IL-10 variants elicit potent immunomodulatory effects at low ligand doses, Science signaling 13 (2020).

R. Götz, T. Kunz, J. Fink, F. Solger, J. Schlegel, J. Seibel, V. Kozjak-Pavlovic, T. Rudel, M. Sauer, Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy, Nature Communications 11, 7478 (2020).

T. Kunz, R. Götz, S. Gao, M. Sauer, V. Kozjak-Pavlovic, Using Expansion Microscopy to Visualize and Characterize the Morphology of Mitochondrial Cristae, Frontiers in cell and developmental biology 8, 617 (2020).

S. Derakhshani, A. Kurz, L. Japtok, F. Schumacher, L. Pilgram, M. Steinke, B. Kleuser, M. Sauer, S. Schneider-Schaulies, E. Avota, Measles Virus Infection Fosters Dendritic Cell Motility in a 3D Environment to Enhance Transmission to Target Cells in the Respiratory Epithelium, Frontiers in immunology 10 (2019).

F. Schueder, J. Stein, F. Stehr, A. Auer, B. Sperl, M. Strauss, P. Schwille, R. Jungmann, An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions, Nature Methods 16, 1101 (2019).

M. Schweikle, S. Bjørnøy, A. van Helvoort, H. Haugen, P. Sikorski, H. Tiainen, Stabilisation of amorphous calcium phosphate in polyethylene glycol hydrogels, Acta Biomaterialia 90, 132 (2019).

Product No.:: AD 643

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ATTO 643

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