ATTO 655
Optical Properties |
||||
λabs |
663 |
nm |
||
εmax |
1.25×105 |
M-1 cm-1 |
||
λfl |
680 |
nm |
||
ηfl |
30 |
% |
||
τfl |
1.8 |
ns |
||
CF260 = ε260/εmax |
0.24 |
|
||
CF280 = ε280/εmax |
0.08 |
|
ATTO 655 belongs together with ATTO 680 und ATTO 700 to a new generation of fluorescent labels. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, exceptionally high stability towards atmospheric ozone, and very good water solubility. Thus ATTO 655 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.
ATTO 655 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 655 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. The fluorescence is excited most efficiently in the range 640 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
Product Sheets
- ATTO 655 product information
- NHS-ester
- maleimide
- iodoacetamide
- phalloidin
- amine
- azide/alkyne
- streptavidin
- tetrazine (MeTet)
Absorption and Emission Spectrum (ASCII)
- ATTO 655 absorption (.txt)
- ATTO 655 emission (.txt)
Absorption and Emission Spectrum (graphic)
- ATTO 655 absorption/fluorescence (.jpg)
Material Safety Data Sheets
- ATTO 655 carboxy MSDS
- ATTO 655 NHS-ester MSDS
- ATTO 655 maleimide MSDS
- ATTO 655 streptavidin MSDS
- ATTO 655 biotin MSDS
- ATTO 655 phalloidin MSDS
- ATTO 655 amine MSDS
- ATTO 655 azide MSDS
- ATTO 655 iodoacetamide MSDS
- ATTO 655 alkyne MSDS
- ATTO 655 cadaverine MSDS
- ATTO 655 MeTet MSDS
Selected Publications for ATTO 655:
B. Dumontel, C. Jiménez-Jiménez, M. Vallet-Regí, M. Manzano, Bioinspired extracellular vesicle-coated silica nanoparticles as selective delivery systems, Materials Today Bio 23, 100850 (2023).
M. Fu, T. Burkart, I. Maryshev, H. Franquelim, A. Merino-Salomón, M. Reverte-López, E. Frey, P. Schwille, Mechanochemical feedback loop drives persistent motion of liposomes, Nature physics 19, 1211 (2023).
A. Gómez-Sánchez, R. Vitale, O. Devos, A. de Juan, C. Ruckebusch, Kernelizing, Analytica Chimica Acta 1273, 341545 (2023).
N. Hartrampf, S. Leitao, N. Winter, H. Toombs-ruane, J. Frank, P. Schwille, D. Trauner, H. Franquelim, Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains, Biophysical Journal 122, 2325 (2023).
L. Kessler, A. Balakrishnan, N. Deußner-Helfmann, Y. Li, M. Mantel, M. Glogger, H.-D. Barth, M. Dietz, M. Heilemann, Self-quenched Fluorophore Dimers for DNA-PAINT and STED Microscopy, Angewandte Chemie International Edition 62, e202307538 (2023).
R. Michenfelder, L. Delafresnaye, V. Truong, C. Barner-Kowollik, H.-A. Wagenknecht, DNA labelling in live cells via visible light-induced [2+2] photocycloaddition, Chemical Communications 59, 4012 (2023).
R. Riera, E. Archontakis, G. Cremers, T. de Greef, P. Zijlstra, L. Albertazzi, Precision and Accuracy of Receptor Quantification on Synthetic and Biological Surfaces Using DNA-PAINT, ACS sensors 8, 80 (2023).
L. Socrier, S. Ahadi, M. Bosse, C. Montag, D. Werz, C. Steinem, Optical Manipulation of Gb3 Enriched Lipid Domains, Chemistry – A European Journal 29, e202202766 (2023).
L. van Buren, G. Koenderink, C. Martinez-Torres, DisGUVery, ACS synthetic biology 12, 120 (2023).
M. Vogt, M. Langecker, M. Gouder, E. Kopperger, F. Rothfischer, F. Simmel, J. List, Storage of mechanical energy in DNA nanorobotics using molecular torsion springs, Nature physics 19, 741 (2023).
C. Zaza, G. Chiarelli, L. Zweifel, M. Pilo-Pais, E. Sisamakis, F. Barachati, F. Stefani, G. Acuna, Super-Resolved FRET Imaging by Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy, Small Methods 7, 2201565 (2023).
M. Alsamsam, A. Kopūstas, M. Jurevičiūtė, M. Tutkus, The miEye, HardwareX 12, e00368 (2022).
A. Arsić, C. Hagemann, N. Stajković, T. Schubert, I. Nikić-Spiegel, Minimal genetically encoded tags for fluorescent protein labeling in living neurons, Nature Communications 13, 1 (2022).
H.-J. Fu, R. Su, L. Luo, Z.-J. Chen, T. Sørensen, N. Hildebrandt, Z.-L. Xu, Rapid and Wash-Free Time-Gated FRET Histamine Assays Using Antibodies and Aptamers, ACS Sensors 7, 1113 (2022).
N. Gimber, S. Strauss, R. Jungmann, J. Schmoranzer, Simultaneous Multicolor DNA-PAINT without Sequential Fluid Exchange Using Spectral Demixing, Nano Letters 22, 2682 (2022).
K. Hübner, M. Raab, J. Bohlen, J. Bauer, P. Tinnefeld, Salt-induced conformational switching of a flat rectangular DNA origami structure, Nanoscale 14, 7898 (2022).
K. Kikuchi, L. Adair, J. Lin, E. New, A. Kaur, Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy, Angewandte Chemie International Edition 62, e202204745 (2023).
S. Kohyama, A. Merino-Salomón, P. Schwille, In vitro assembly, positioning and contraction of a division ring in minimal cells, Nature Communications 13, 1 (2022).
M. Malle, P. Löffler, S. Bohr, M. Sletfjerding, N. Risgaard, S. Jensen, M. Zhang, P. Hedegård, S. Vogel, N. Hatzakis, Single-particle combinatorial multiplexed liposome fusion mediated by DNA, Nature Chemistry 14, 558 (2022).
N. Niranjan Dhanasekar, D. Thiyagarajan, D. Bhatia, DNA Origami in the Quest for Membrane Piercing, Chemistry – An Asian Journal 17, e202200591 (2022).
S. Nooteboom, Y. Wang, S. Dey, P. Zijlstra, Real-Time Interfacial Nanothermometry Using DNA-PAINT Microscopy, Small 18, 2201602 (2022).
L. Ostersehlt, D. Jans, A. Wittek, J. Keller-Findeisen, K. Inamdar, S. Sahl, S. Hell, S. Jakobs, DNA-PAINT MINFLUX nanoscopy, Nature Methods 19, 1072 (2022).
A.-K. Pumm, W. Engelen, E. Kopperger, J. Isensee, M. Vogt, V. Kozina, M. Kube, M. Honemann, E. Bertosin, M. Langecker, R. Golestanian, F. Simmel, H. Dietz, A DNA origami rotary ratchet motor, Nature 607, 492 (2022).
R. Riera, E. Archontakis, G. Cremers, T. de Greef, P. Zijlstra, L. Albertazzi, Precision and Accuracy of Receptor Quantification on Synthetic and Biological Surfaces Using DNA-PAINT, ACS sensors 8, 80 (2023).
D. Sakhapov, I. Gregor, N. Karedla, J. Enderlein, Measuring Photophysical Transition Rates with Fluorescence Correlation Spectroscopy and Antibunching, The journal of physical chemistry letters 13, 4823 (2022).
E. Taylor, F. Abounorinejad, E. Jacobo, A. Dixon, K. Lam, J. Brozik, Photophysical Rate Constants and Oxygen Dependence for Si and Ge Rhodamine Zwitterions, The journal of physical chemistry. A 127, 851 (2023).
M. Thommen, A. Draycheva, M. Rodnina, Ribosome selectivity and nascent chain context in modulating the incorporation of fluorescent non-canonical amino acid into proteins, Scientific Reports 12, 12848 (2022).
A. Villamil Giraldo, S. Mannsverk, P. Kasson, Measuring single-virus fusion kinetics using an assay for nucleic acid exposure, Biophysical Journal 121, 4467 (2022).
G. Virdi, M. Choi, J. Evans, Z. Yao, D. Athauda, S. Strohbuecker, R. Nirujogi, A. Wernick, N. Pelegrina-Hidalgo, C. Leighton, R. Saleeb, O. Kopach, H. Alrashidi, D. Melandri, J. Perez-Lloret, P. Angelova, S. Sylantyev, S. Eaton, S. Heales, D. Rusakov, D. Alessi, T. Kunath, M. Horrocks, A. Abramov, R. Patani, S. Gandhi, Protein aggregation and calcium dysregulation are hallmarks of familial Parkinson’s disease in midbrain dopaminergic neurons, npj Parkinson's Disease 8, 1 (2022).
M. Zhang, H. Pinholt, X. Zhou, S. Bohr, L. Banetta, A. Zaccone, V. Foderà, N. Hatzakis, Direct observation of heterogeneous formation of amyloid spherulites in real-time by super-resolution microscopy, Communications Biology 5, 1 (2022).
H. Abdel-Bar, A. Walters, J. Wang, K. Al-Jamal, Combinatory Delivery of Etoposide and siCD47 in a Lipid Polymer Hybrid Delays Lung Tumor Growth in an Experimental Melanoma Lung Metastatic Model, Advanced Healthcare Materials 10, 2001853 (2021).
G. Berselli, A. Gimenez, A. O’Connor, T. Keyes, Robust Photoelectric Biomolecular Switch at a Microcavity-Supported Lipid Bilayer, ACS Applied Materials & Interfaces 13, 29158 (2021).
T. Diederichs, K. Ahmad, J. Burns, Q. Nguyen, Z. Siwy, M. Tornow, P. Coveney, R. Tampé, S. Howorka, Principles of Small-Molecule Transport through Synthetic Nanopores, ACS Nano 15, 16194 (2021).
N. Eerqing, S. Subramanian, J. Rubio, T. Lutz, H.-Y. Wu, J. Anders, C. Soeller, F. Vollmer, Comparing Transient Oligonucleotide Hybridization Kinetics Using DNA-PAINT and Optoplasmonic Single-Molecule Sensing on Gold Nanorods, ACS Photonics 8, 2882 (2021).
H.-J. Fu, R. Su, L. Luo, Z.-J. Chen, T. Sørensen, N. Hildebrandt, Z.-L. Xu, Rapid and Wash-Free Time-Gated FRET Histamine Assays Using Antibodies and Aptamers, ACS Sensors 7, 1113 (2022).
N. Gimber, S. Strauss, R. Jungmann, J. Schmoranzer, Simultaneous Multicolor DNA-PAINT without Sequential Fluid Exchange Using Spectral Demixing, Nano Letters 22, 2682 (2022).
A. Guha, M. McGuire, G. Leriche, J. Yang, M. Mayer, A single-liposome assay that enables temperature-dependent measurement of proton permeability of extremophile-inspired lipid membranes, Biochimica et Biophysica Acta (BBA) - Biomembranes 1863, 183567 (2021).
T. Heermann, H. Franquelim, P. Glock, L. Harrington, P. Schwille, Probing Biomolecular Interactions by a Pattern-Forming Peptide-Conjugate Sensor, Bioconjugate Chemistry 32, 172 (2021).
M. Hurley, T. Sheard, R. Norman, H. Kirton, S. Shah, E. Pervolaraki, Z. Yang, N. Gamper, E. White, D. Steele, I. Jayasinghe, A correlative super-resolution protocol to visualise structural underpinnings of fast second-messenger signalling in primary cell types, Methods 193, 27 (2021).
K. Kawai, M. Fujitsuka, A. Maruyama, Single-Molecule Study of Redox Reaction Kinetics by Observing Fluorescence Blinking, Accounts of Chemical Research 54, 1001 (2021).
M. Lelek, M. Gyparaki, G. Beliu, F. Schueder, J. Griffié, S. Manley, R. Jungmann, M. Sauer, M. Lakadamyali, C. Zimmer, Single-molecule localization microscopy, Nature Reviews Methods Primers 1, 1 (2021).
D. Li, M. Ahmed, A. Khan, L. Xu, A. Walters, B. Ballesteros, K. Al-Jamal, Tailoring the Architecture of Cationic Polymer Brush-Modified Carbon Nanotubes for Efficient siRNA Delivery in Cancer Immunotherapy, ACS Applied Materials & Interfaces 13, 30284 (2021).
D. Ramirez-Diaz, A. Merino-Salomón, F. Meyer, M. Heymann, G. Rivas, M. Bramkamp, P. Schwille, FtsZ induces membrane deformations via torsional stress upon GTP hydrolysis, Nature Communications 12, 1 (2021).
T. Rudack, C. Teuber, M. Scherlo, J. Güldenhaupt, J. Schartner, M. Lübben, J. Klare, K. Gerwert, C. Kötting, The Ras dimer structure, Chemical Science 12, 8178 (2021).
A. Salter, A. Rajan, J. Kennedy, R. Ivey, S. Shelby, I. Leung, M. Templeton, V. Muhunthan, V. Voillet, D. Sommermeyer, J. Whiteaker, R. Gottardo, S. Veatch, A. Paulovich, S. Riddell, Comparative analysis of TCR and CAR signaling informs CAR designs with superior antigen sensitivity and in vivo function, Science signaling 14 (2021).
M. Schröder, M.-L. Harwardt, J. Rahm, Y. Li, P. Freund, M. Dietz, M. Heilemann, Imaging the fibroblast growth factor receptor network on the plasma membrane with DNA-assisted single-molecule super-resolution microscopy, Methods 193, 38 (2021).
K. Spustova, E. Köksal, A. Ainla, I. Gözen, Subcompartmentalization and Pseudo-Division of Model Protocells, Small 17, 2005320 (2021).
S. Stealey, A. Gaharwar, N. Pozzi, S. Zustiak, Development of Nanosilicate–Hydrogel Composites for Sustained Delivery of Charged Biopharmaceutics, ACS Applied Materials & Interfaces 13, 27880 (2021).
X. Tan, K. Welsher, Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy**, Angewandte Chemie International Edition 60, 22359 (2021).
M. Wester, D. Schodt, H. Mazloom-Farsibaf, M. Fazel, S. Pallikkuth, K. Lidke, Robust, fiducial-free drift correction for super-resolution imaging, Scientific Reports 11, 1 (2021).
T. Andresen, J. Larsen, Compositional inhomogeneity of drug delivery liposomes quantified at the single liposome level, Acta Biomaterialia 118, 207 (2020).
P. Blond, R. Bevernaegie, L. Troian-Gautier, C. Lagrost, J. Hubert, F. Reniers, V. Raussens, I. Jabin, Ready-to-Use Germanium Surfaces for the Development of FTIR-Based Biosensors for Proteins, Langmuir 36, 12068 (2020).
B. Brownlee, J. Claussen, B. Iverson, 3D Interdigitated Vertically Aligned Carbon Nanotube Electrodes for Electrochemical Impedimetric Biosensing, ACS Applied Nano Materials 3, 10166 (2020).
K. Cervantes-Salguero, M. Freeley, J. Chávez, M. Palma, Single-molecule DNA origami aptasensors for real-time biomarker detection, Journal of Materials Chemistry B 8, 6352 (2020).
P. Franz, V. Gassl, A. Topf, L. Eckelmann, B. Iorga, G. Tsiavaliaris, A thermophoresis–based biosensor for real–time detection of inorganic phosphate during enzymatic reactions, Biosensors and Bioelectronics 169, 112616 (2020).
Hedvig Tamman, Katleen Van Nerom, Hiraku Takada, Niels Vandenberk, Daniel Scholl, Yury Polikanov, Johan Hofkens, Ariel Talavera, Vasili Hauryliuk, Jelle Hendrix, Abel Garcia-Pino, A nucleotide-switch mechanism mediates opposing catalytic activities of Rel enzymes, Nature Chemical Biology 16, 834 (2020).
T. Heermann, B. Ramm, S. Glaser, P. Schwille, Local Self-Enhancement of MinD Membrane Binding in Min Protein Pattern Formation, Journal of Molecular Biology 432, 3191 (2020).
Jan Möller, Ali Isbilir, Titiwat Sungkaworn, Brendan Osberg, Christos Karathanasis, Vikram Sunkara, Eugene O. Grushevskyi, Andreas Bock, Paolo Annibale, Mike Heilemann, Christof Schütte, Martin J. Lohse, Single-molecule analysis reveals agonist-specific dimer formation of µ-opioid receptors, Nature Chemical Biology 16, 946 (2020).
A. Jimenez, K. Friedl, C. Leterrier, About samples, giving examples, Methods 174, 100 (2020).
S. Li, J. He, Q.-H. Xu, Aggregation of Metal-Nanoparticle-Induced Fluorescence Enhancement and Its Application in Sensing, ACS Omega 5, 41 (2020).
R. Lin, A. Clowsley, T. Lutz, D. Baddeley, C. Soeller, 3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples, Methods 174, 56 (2020).
E. Mercier, W. Wintermeyer, M. Rodnina, Co-translational insertion and topogenesis of bacterial membrane proteins monitored in real time, The EMBO Journal 39, e104054 (2020).
M. Scheckenbach, T. Schubert, C. Forthmann, V. Glembockyte, P. Tinnefeld, Self-Regeneration and Self-Healing in DNA Origami Nanostructures, Angewandte Chemie International Edition (2020).
M. Schröder, M.-L. Harwardt, J. Rahm, Y. Li, P. Freund, M. Dietz, M. Heilemann, Imaging the fibroblast growth factor receptor network on the plasma membrane with DNA-assisted single-molecule super-resolution microscopy, Methods (2020).
S. Simoncelli, J. Griffié, D. Williamson, J. Bibby, C. Bray, R. Zamoyska, A. Cope, D. Owen, Multi-color Molecular Visualization of Signaling Proteins Reveals How C-Terminal Src Kinase Nanoclusters Regulate T Cell Receptor Activation, Cell Reports 33, 108523 (2020).
C. Solís, J. Robinson, Cardiac troponin and tropomyosin bind to F-actin cooperatively, as revealed by fluorescence microscopy, FEBS Open Bio 10, 1362 (2020).
J. Thiele, D. Helmerich, N. Oleksiievets, R. Tsukanov, E. Butkevich, M. Sauer, O. Nevskyi, J. Enderlein, Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy, ACS Nano 14, 14190 (2020).
H. Kim, H. Choi, J. Eom, Y. Choi, Mini-Platform for Off-On Near-Infrared Fluorescence Imaging Using Peptide-Targeting Ligands, Bioconjugate Chemistry 31, 721 (2020).
A. Aliyan, N. Cook, A. Martí, Interrogating Amyloid Aggregates using Fluorescent Probes, Chemical Reviews 119, 11819 (2019).
S. Bohr, P. Lund, A. Kallenbach, H. Pinholt, J. Thomsen, L. Iversen, A. Svendsen, S. Christensen, N. Hatzakis, Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition, Scientific Reports 9, 1 (2019).
Y. Chen, W. Xie, A. Glaser, N. Reder, C. Mao, S. Dintzis, J. Vaughan, J. Liu, Rapid pathology of lumpectomy margins with open-top light-sheet (OTLS) microscopy, Biomedical Optics Express 10, 1257 (2019).
A. Ghosh, A. Sharma, A. Chizhik, S. Isbaner, D. Ruhlandt, R. Tsukanov, I. Gregor, N. Karedla, J. Enderlein, Graphene-based metal-induced energy transfer for sub-nanometre optical localization, Nature Photonics 313, 1642 (2019).
M. Gómez-Castaño, A. Redondo-Cubero, L. Buisson, J. Pau, A. Mihi, S. Ravaine, R. Vallée, A. Nitzan, M. Sukharev, Energy Transfer and Interference by Collective Electromagnetic Coupling, Nano letters 19, 5790 (2019).
V. González-Pedro, M. Calvo, H. Míguez, Á. Maquieira, Nanoparticle Bragg reflectors, Biosensors and Bioelectronics: X 1, 100012 (2019).
I. Gregor, A. Chizhik, N. Karedla, J. Enderlein, Metal-induced energy transfer, Nanophotonics 8, 1689 (2019).
R. Gruber, T. Mondal, A. Horovitz, GroEL Allostery Illuminated by a Relationship between the Hill Coefficient and the MWC Model, Biophysical Journal 117, 1915 (2019).
Y. Han, M. Li, M. Zhang, X. Huang, L. Chen, X. Hao, C. Kuang, X. Liu, Y.‐H. Zhang, Cell‐permeable organic fluorescent probes for live‐cell super‐resolution imaging of actin filaments, Journal of Chemical Technology & Biotechnology 94, 2040 (2019).
B. Iyisan, K. Landfester, Modular Approach for the Design of Smart Polymeric Nanocapsules, Macromolecular Rapid Communications 40 (2019).
M. Kol, B. Williams, H. Toombs-ruane, H. Franquelim, S. Korneev, C. Schroeer, P. Schwille, D. Trauner, J. Holthuis, J. Frank, Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides, eLife 8, e43230 (2019).
S. Li, J. He, Q.-H. Xu, Aggregation of Metal-Nanoparticle-Induced Fluorescence Enhancement and Its Application in Sensing, ACS Omega 5, 41 (2020).
D. Nieves, G. Hilzenrat, J. Tran, Z. Yang, H. MacRae, M. Baker, J. Gooding, K. Gaus, tagPAINT, Royal Society Open Science 6, 191268 (2019).
R. Oliveira-Silva, M. Sousa-Jerónimo, D. Botequim, N. Silva, D. Prazeres, P. Paulo, Density Gradient Selection of Colloidal Silver Nanotriangles for Assembling Dye-Particle Plasmophores, Nanomaterials 9, 893 (2019).
S. Park, M. Reyer, E. McLean, W. Liu, J. Fei, An Improved Method for Bacterial Immunofluorescence Staining To Eliminate Antibody Exclusion from the Fixed Nucleoid, Biochemistry 58, 4457 (2019).
M. Philippi, C. You, C. Richter, M. Schmidt, J. Thien, D. Liße, J. Wollschläger, J. Piehler, M. Steinhart, Close-packed silane nanodot arrays by capillary nanostamping coupled with heterocyclic silane ring opening, RSC Advances 9, 24742 (2019).
S. Ramadurai, A. Kohut, N. Sarangi, O. Zholobko, V. Baulin, A. Voronov, T. Keyes, Macromolecular inversion-driven polymer insertion into model lipid bilayer membranes, Journal of Colloid and Interface Science 542, 483 (2019).
S. Samanta, W. Gong, W. Li, A. Sharma, I. Shim, W. Zhang, P. Das, W. Pan, L. Liu, Z. Yang, J. Qu, J. Kim, Organic fluorescent probes for stochastic optical reconstruction microscopy (STORM), Coordination Chemistry Reviews 380, 17 (2019).
S. Shah, A. Dubey, J. Reif, Improved Optical Multiplexing with Temporal DNA Barcodes, ACS synthetic biology 8, 1100 (2019).
S. Sheth, E. Barnard, B. Hyatt, M. Rathinam, S. Zustiak, Predicting Drug Release From Degradable Hydrogels Using Fluorescence Correlation Spectroscopy and Mathematical Modeling, Frontiers in Bioengineering and Biotechnology 7, 410 (2019).
R. Thomsen, M. Galsgaard Malle, A. Hauge Okholm, S. Krishnan, S. Bohr, R. Schøler Sørensen, O. Ries, S. Vogel, F. Simmel, N. Hatzakis, J. Kjems, A large size-selective DNA nanopore with sensing applications, Nature Communications 10, 1 (2019).
S. Wang, Single Molecule RNA FISH (smFISH) in Whole‐Mount Mouse Embryonic Organs, Current protocols in cell biology 83 (2019).
W. Xie, Y. Chen, Y. Wang, L. Wei, C. Yin, A. Glaser, M. Fauver, E. Seibel, S. Dintzis, J. Vaughan, N. Reder, J. Liu, Microscopy with ultraviolet surface excitation for wide-area pathology of breast surgical margins, Journal of biomedical optics 24, 1 (2019).
S. Kempter, A. Khmelinskaia, M. Strauss, P. Schwille, R. Jungmann, T. Liedl, W. Bae, Single Particle Tracking and Super-Resolution Imaging of Membrane-Assisted Stop-and-Go Diffusion and Lattice Assembly of DNA Origami, ACS Nano 13, 996 (2019).
O. Wade, J. Woehrstein, P. Nickels, S. Strauss, F. Stehr, J. Stein, F. Schueder, M. Strauss, M. Ganji, J. Schnitzbauer, H. Grabmayr, P. Yin, P. Schwille, R. Jungmann, 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics, Nano letters (2019).
A. Andreoni, S. Sen, P.-L. Hagedoorn, W. Buma, T. Aartsma, G. Canters, Fluorescence Correlation Spectroscopy of Labeled Azurin Reveals Photoinduced Electron Transfer between Label and Cu Center, Chemistry (Weinheim an der Bergstrasse, Germany) 24, 646 (2018).
T. Diederichs, Q. Nguyen, M. Urban, R. Tampé, M. Tornow, Transparent Nanopore Cavity Arrays Enable Highly Parallelized Optical Studies of Single Membrane Proteins on Chip, Nano letters 18, 3901 (2018).
V. Fabricius, J. Lefèbre, H. Geertsema, S. Marino, H. Ewers, Rapid and efficient C-terminal labeling of nanobodies for DNA-PAINT, Journal of Physics D: Applied Physics 51, 474005 (2018).
C. Hulleman, W. Li, I. Gregor, B. Rieger, J. Enderlein, Photon Yield Enhancement of Red Fluorophores at Cryogenic Temperatures, ChemPhysChem 19, 1774 (2018).
N. Karedla, A. Chizhik, S. Stein, D. Ruhlandt, I. Gregor, A. Chizhik, J. Enderlein, Three-dimensional single-molecule localization with nanometer accuracy using Metal-Induced Energy Transfer (MIET) imaging, The Journal of Chemical Physics 148, 204201 (2018).
A. Khmelinskaia, J. Mücksch, F. Conci, G. Chwastek, P. Schwille, FCS Analysis of Protein Mobility on Lipid Monolayers, Biophysical Journal 114, 2444 (2018).
C. Kohout, C. Santi, L. Polito, Anisotropic Gold Nanoparticles in Biomedical Applications, International journal of molecular sciences 19, 3385 (2018).
E. Kopperger, J. List, S. Madhira, F. Rothfischer, D. Lamb, F. Simmel, A self-assembled nanoscale robotic arm controlled by electric fields, Science 359, 296 (2018).
G. Li, K. Row, Applications of Magnetic Molecularly Imprinted Polymers (MMIPs) in the Separation and Purification Fields, Chromatographia 81, 73 (2018).
M. Masureel, Y. Zou, L.-P. Picard, E. van der Westhuizen, J. Mahoney, Rodrigues, João P. G. L. M., T. Mildorf, R. Dror, D. Shaw, M. Bouvier, E. Pardon, J. Steyaert, R. Sunahara, W. Weis, C. Zhang, B. Kobilka, Structural insights into binding specificity, efficacy and bias of a β 2 AR partial agonist, Nature chemical biology 14, 1059 (2018).
P. Paulo, D. Botequim, A. Jóskowiak, S. Martins, D. Prazeres, P. Zijlstra, S. Costa, Enhanced Fluorescence of a Dye on DNA-Assembled Gold Nanodimers Discriminated by Lifetime Correlation Spectroscopy, The Journal of Physical Chemistry C 122, 10971 (2018).
R. Sasmal, N. Das Saha, M. Pahwa, S. Rao, D. Joshi, M. Inamdar, V. Sheeba, S. Agasti, Synthetic Host-Guest Assembly in Cells and Tissues, Analytical chemistry 90, 11305 (2018).
P. Taborsky, J. Kucera, J. Jurica, O. Pes, Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography, Journal of Chromatography B 1092, 7 (2018).
N. Vandenberk, A. Barth, D. Borrenberghs, J. Hofkens, J. Hendrix, Evaluation of Blue and Far-Red Dye Pairs in Single-Molecule Förster Resonance Energy Transfer Experiments, The Journal of Physical Chemistry B 122, 4249 (2018).
D. Virant, B. Traenkle, J. Maier, P. Kaiser, M. Bodenhöfer, C. Schmees, I. Vojnovic, B. Pisak-Lukáts, U. Endesfelder, U. Rothbauer, A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging, Nature Communications 9, 930 (2018).
J. Wang, Z. Zhou, F. Zhang, H. Xu, W. Chen, T. Jiang, A novel nanocomposite based on fluorescent turn-on gold nanostars for near-infrared photothermal therapy and self-theranostic caspase-3 imaging of glioblastoma tumor cell, Colloids and Surfaces B: Biointerfaces 170, 303 (2018).
G. Ashdown, G. Burn, D. Williamson, E. Pandžić, R. Peters, M. Holden, H. Ewers, L. Shao, P. Wiseman, D. Owen, Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse, Biophysical journal 112, 1703 (2017).
B. Eggart, M. Scheible, C. Forthmann, Beyond the Diffraction Limit, Optik & Photonik 12, 26 (2017).
M. Grieb, A. Nivina, B. Cheeseman, A. Hartmann, D. Mazel, M. Schlierf, Dynamic stepwise opening of integron attC DNA hairpins by SSB prevents toxicity and ensures functionality, Nucleic Acids Research 45, 10555 (2017).
E. Jain, S. Sheth, A. Dunn, S. Zustiak, S. Sell, Sustained release of multicomponent platelet-rich plasma proteins from hydrolytically degradable PEG hydrogels, Journal of biomedical materials research. Part A 105, 3304 (2017).
F. Liang, G. Lindgren, A. Lin, E. Thompson, S. Ols, J. Röhss, S. John, K. Hassett, O. Yuzhakov, K. Bahl, L. Brito, H. Salter, G. Ciaramella, K. Loré, Efficient Targeting and Activation of Antigen-Presenting Cells In Vivo after Modified mRNA Vaccine Administration in Rhesus Macaques, Molecular therapy : the journal of the American Society of Gene Therapy 25, 2635 (2017).
C.-W. Lin, B. Mensa, M. Barniol-Xicota, W. DeGrado, F. Gai, Activation pH and Gating Dynamics of Influenza A M2 Proton Channel Revealed by Single-Molecule Spectroscopy, Angewandte Chemie 129, 5367 (2017).
S. Ramadurai, M. Werner, N. Slater, A. Martin, V. Baulin, T. Keyes, Dynamic studies of the interaction of a pH responsive, amphiphilic polymer with a DOPC lipid membrane, Soft Matter 13, 3690 (2017).
F. Schneider, D. Ruhlandt, I. Gregor, J. Enderlein, A. Chizhik, Quantum Yield Measurements of Fluorophores in Lipid Bilayers Using a Plasmonic Nanocavity, The journal of physical chemistry letters 8, 1472 (2017).
A. Sharma, J. Enderlein, M. Kumbhakar, Photon Antibunching Reveals Static and Dynamic Quenching Interaction of Tryptophan with Atto-655, The journal of physical chemistry letters 8, 5821 (2017).
J. Werbin, M. Avendaño, V. Becker, R. Jungmann, P. Yin, G. Danuser, P. Sorger, Multiplexed Exchange-PAINT imaging reveals ligand-dependent EGFR and Met interactions in the plasma membrane, Scientific Reports 7, 12150 (2017).
G. Arrabito, F. Cavaleri, V. Montalbano, V. Vetri, M. Leone, B. Pignataro, Monitoring few molecular binding events in scalable confined aqueous compartments by raster image correlation spectroscopy (CADRICS), Lab on a Chip 16, 4666 (2016).
N. Dimitriadis, B. Grychtol, L. Maertins, T. Behr, G. Themelis, N. Deliolanis, Simultaneous real-time multicomponent fluorescence and reflectance imaging method for fluorescence-guided surgery, Optics Letters 41, 1173 (2016).
B. Ding, M. Hilaire, F. Gai, Infrared and Fluorescence Assessment of Protein Dynamics: From Folding to Function, The Journal of Physical Chemistry B 120, 5103 (2016).
J. Frank, H. Franquelim, P. Schwille, D. Trauner, Optical Control of Lipid Rafts with Photoswitchable Ceramides, Journal of the American Chemical Society 138, 12981 (2016).
S. Isbaner, N. Karedla, D. Ruhlandt, S. Stein, A. Chizhik, I. Gregor, J. Enderlein, Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging, Optics Express 24, 9429 (2016).
R. Jungmann, M. Avendaño, M. Dai, J. Woehrstein, S. Agasti, Z. Feiger, A. Rodal, P. Yin, Quantitative super-resolution imaging with qPAINT, Nature Methods 13, 439 (2016).
J. Molle, M. Raab, S. Holzmeister, D. Schmitt-Monreal, D. Grohmann, Z. He, P. Tinnefeld, Superresolution microscopy with transient binding, Current Opinion in Biotechnology 39, 8 (2016).
T. Niehörster, A. Löschberger, I. Gregor, B. Krämer, H.-J. Rahn, M. Patting, F. Koberling, J. Enderlein, M. Sauer, Multi-target spectrally resolved fluorescence lifetime imaging microscopy, Nature Methods 13, 257 (2016).
I. Nikic, G. Estrada Girona, J. Kang, G. Paci, S. Mikhaleva, C. Koehler, N. Shymanska, C. Ventura Santos, D. Spitz, E. Lemke, Verbesserte Erweiterung des eukaryotischen genetischen Codes für seitenspezifische, hochauflösende Click-PAINT-Mikroskopie, Angewandte Chemie 128, 16406 (2016).
B. Pradhan, S. Khatua, A. Gupta, T. Aartsma, G. Canters, M. Orrit, Gold-Nanorod-Enhanced Fluorescence Correlation Spectroscopy of Fluorophores with High Quantum Yield in Lipid Bilayers, The Journal of Physical Chemistry C 120, 25996 (2016).
S. Hennig, S. van de Linde et al., Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes, Nano Lett. 15, 1374 (2015).
N. Karedla, A.I. Chizhik et al., Single-Molecule Metal-Induced Energy Transfer (smMIET): Resolving Nanometer Distances at the Single-Molecule Level, ChemPhysChem 15, 705 (2014).
P. Holzmeister, G.P. Acuna et al., Breaking the concentration limit of optical single-molecule detection, Chem. Soc. Rev. 43, 1014 (2014).
R.F. Correia, M.I. Viseu, S.M. Andrade, Aggregation/Disaggregation of Chlorophyll a in Model Phospholipid−Detergent Vesicles and Micelles, Photochem. Photobiol. Sci. 13, 907 (2014).
C. You, C.P. Richter et al., Dynamic Submicroscopic Signaling Zones Revealed by Pair Correlation Tracking and Localization Microscopy, Anal. Chem. 86, 8593 (2014).
C. Annink, R. Gill, Nanoparticle Aggregate-Based Fluorescence Enhancement for Highly Sensitive and Reproducible Detection of DNA, Part. Part. Syst. Charact. 31, 943 (2014).
S. Ghosh, M. Kumbhakar et al., Single-molecule fluorescence inside solid-state nanochannels, Proc. SPIE 8950, 89508 (2014).
T. Steinberger, R. Machan, M. Hof, Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes, Meth. Mol. Biol. 1076, 617 (2014).
E. Ploetz, B. Visser et al., Selective functionalization of patterned glass surfaces, J. Mater. Chem. B 2, 2606 (2014).
Y.M. Shlyapnikov, E.A. Shlyapnikova, V.N. Morozov, Carboxymethyl Cellulose Film as a Substrate for Microarray Fabrication, Anal. Chem. 86, 2082 (2014).
M.B. Scheible, G. Pardatscher et al., Single Molecule Characterization of DNA Binding and Strand Displacement Reactions on Lithographic DNA Origami Microarrays, Nano Lett. 14, 1627 (2014).
Y. Zhang, S. Yuan, Ultrafast Fluorescence Quenching Dynamics of Atto655 in the Presence of N Acetyltyrosine and N Acetyltryptophan in Aqueous Solution: Proton-Coupled Electron Transfer versus Electron Transfer, J. Phys. Chem. B 117, 7308 (2013).
S.K. Jorgensen, N.S. Hatzakis, Insights in enzyme functional dynamics and activity regulation by single molecule studies, Biophys. Rev. Lett. 8, 137 (2013).
H. Bao, F. Duong, ATP Alone Triggers the Outward Facing Conformation of the Maltose ATP-binding Cassette Transporter, J. Biol. Chem. 288, 3439 (2013).
C. Dong, B. Chowdhury, J. Irudayaraj, Probing site-exclusive binding of aqueous QDs and their organelle-dependent dynamics in live cells by single molecule spectroscopy, Analyst 138, 2871 (2013).
K. Kawai, T. Majima, A. Maruyama, Detection of Single-Nucleotide Variations by Monitoring the Blinking of Fluorescence Induced by Charge Transfer in DNA, ChemBioChem 14, 1430 (2013).
Z. Truan, L. Trancon Diez et al., Quantitative morphological analysis of arrestin2 clustering upon G protein-coupled receptor stimulation by super-resolution microscopy, J. Struct. Biol. 184, 329 (2013).
O. Schulz, C. Pieper et al., Resolution doubling in fluorescence microscopy with confocal spinning-disk image scanning microscopy, PNAS 110, 21000 (2013).
Z. Chen, C. Jing et al., Second-Generation Covalent TMP-Tag for Live Cell Imaging, J. Am. Chem. Soc. 134, 13692 (2012).
F. Heinemann, V. Betaneli et al., Quantifying Lipid Diffusion by Fluorescence Correlation Spectroscopy: A Critical Treatise, Langmuir 28, 13395 (2012).
S. Preus, L.M. Wilhelmsson, Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids, ChemBioChem 13, 1990 (2012).
W. Slingenbergh, S.K. de Boer et al., Selective Functionalization of Tailored Nanostructures, ACS Nano 6, 9214 (2012).
R. Gill, L. Tian et al., Silver Nanoparticle Aggregates as Highly Efficient Plasmonic Antennas for Fluorescence Enhancement, J. Phys. Chem. C 116, 16687 (2012).
S. Roizard, C. Danelon et al., Activation of G-Protein-Coupled Receptors in Cell-Derived Plasma Membranes Supported on Porous Beads, J. Am. Chem. Soc. 133, 16868 (2011).
E. Sherman, G. Haran, Fluorescence Correlation Spectroscopy of Fast Chain Dynamics within Denatured Protein L, ChemPhysChem 12, 696 (2011).
B. Xing, T. Jiang, Molecular Interactions between Glycopeptide Vancomycin and Bacterial Cell Wall Peptide Analogues, Chem. Eur. J. 17, 14170 (2011).
R. Abe, H. Ohashi et al., “Quenchbodies”: Quench-Based Antibody Probes That Show Antigen-Dependent Fluorescence, J. Am. Chem. Soc. 133, 17386 (2011).
W.C. Duim, B. Chen et al., Sub-Diffraction Imaging of Huntingtin Protein Aggregates by Fluorescence Blink-Microscopy and Atomic Force Microscopy, ChemPhysChem 12, 2387 (2011).
E. Cabane, V. Malinova et al., Photoresponsive polymersomes as smart, triggerable nanocarriers, Soft Matter 7, 9167 (2011).
J.G. Bruno, M.A. Zuninga et al., Development of Naturally Selected and Molecularly Engineered Intrachain and Competitive FRET-Aptamers and Aptamer Beacons, Comb. Chem. High Throughput Screen. 14, 622 (2011).
T. Gao, C.D. Blanchette et al., Characterizing diffusion dynamics of a membrane protein associated with nanolipoproteins using fluorescence correlation spectroscopy, Protein Sci. 20, 437 (2011).
G. Almer, M. Saba-Lepek et al., Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions, Biologics: Targets and Therapy 5, 95 (2011).
R. Sun, R. Lu, A. Yu, Structural Heterogeneity in the Collision Complex between Organic Dyes and Tryptophan in Aqueous Solution, J. Phys. Chem. B 115, 660 (2011).
K. Kawai, E. Matsutani et al., Probing the Charge-Transfer Dynamics in DNA at the Single-Molecule Level, J. Am. Chem. Soc. 133, 15568 (2011).
A. J. Garcia-Saez, S.B. Buschhorn et al., Oligomerization and Pore Formation by Equinatoxin II Inhibit Endocytosis and Lead to Plasma Membrane Reorganization, J. Biol. Chem. 286, 3768 (2011).
P. J. Yoo, W.-S. Choe et al., Physically and Chemically Confined Nano-Patters of Proteins and Bacteriophages, Mater. Express 1, 51 (2011).
J. M. G. Rogers, A. L. Polishchuk et al., Photoinduced Electron Transfer and Fluorophore Motion as a Probe of the Conformational Dynamics of Membrane Proteins: Application to the Influenza A M2 Proton Channel, Langmuir 27, 3815 (2011).
R. Zhu, X. Li et al., Photophysical Properties of ATTO655 Dye in the Presence of Guanosine and Tryptophan in Aqueous Solution, J. Phys. Chem. B 115, 5001 (2011).
S. Rajagopalan, F. Huang, A. R. Fersht, Single-Molecule characterization of oligomerization kinetics and equilibria of the tumor suppressor p53, Nucleic Acids Res. 39, 2294 (2011).
C. D. James, J. McClain et al., High-efficiency magnetic particle focusing using dielectrophoresis and magnetophoresis in a microfluidic device, J. Micromech. Microeng. 20, 045015 (2010).
T. Kottke, S. van de Linde et al., Identification of the Product of Photoswitching of an Oxazine Fluorophore Using Fourier Transform Infrared Difference Spectroscopy, J. Phys. Chem. Lett. 1, 3156 (2010).
S. Rüttinger, P. Kapusta et al., On the Resolution Capabilities and Limits of Fluorescence Lifetime Correlation Spectroscopy (FLCS) Measurements, J. Fluoresc. 20, 105 (2010).
A. V. Patil, J. J. Davis, Visualizing and Tuning Thermodynamic Dispersion in Metalloprotein Monolayers, J. Am. Chem. Soc. 132, 16938 (2010).
R. Wombacher, M. Heidbreder et al., Live-cell super-resolution imaging with trimethoprim conjugates, Nature Methods 7, 717 (2010).
A. Pelah, A. Bharde, T.M. Jovin, Protein manipulation by stimuli-responsive polymers encapsulated in erythrocyte ghosts, Soft Matter 5, 1006 (2009).
Y. Elkasabi, J. Lahann, Vapor-Based Polymer Gradients, Macromol. Rapid Comm. 30, 57 (2009).
S. van der Linde et al., Multicolor Photoswitching Microscopy for Subdifraction-Resolution Fluorescence Imaging, Photochem. Photobiol. Sci. 2009, doi: 10.1039/b822533h
H. Tezval et al., The Involvement of Altered Corticotropin Releasing Factor Receptor 2 Expression in Prostate Cancer Due to Alteration of Anti-Angiogenic Signaling Pathways, Prostate 69, 443 (2009).
S. van der Linde, M. Heilemann, M. Sauer, Photoswitching microscopy with standard fluorophores, Appl. Phys. B 93, 725 (2008).
R. Priya et al., Low resolution structure of subunit b(b22-156) of Escherichia coli F1FO ATP synthase in solution and the b-d assembly, J. Bioenerg. Biomembr. 40, 245 (2008).
Y. Korlann, T. Dertinger, X. Michalet, S. Weiss, J. Enderlein, Measuring diffusion with polarization-modulation dual-focus fluorescence correlations spectroscopy, Optics Express 16, 14609 (2008).
H.-Y. Chen, A.A. McClelland, Z. Chen, J. Lahann, Solventless Adhesive Bonding Using Reactive Polymer Coatings, Anal. Chem. 80, 4119 (2008).
G.A. Frank, Y. Kipnis, E. Smolensky, S.S. Daube, A. Horovitz et al., Design of an Optical Swich for Studying Conformational Dynamics in Individual Molecules of GroEL, Bioconjugate Chem. 19, 1339 (2008).
S. Kutzetsova, G. Zauner, T.J. Aartsma et al., The enzyme mechanism of nitrile reductase studied at single-molecule level, PNAS 105, 3250 (2008).
S. Rüttinger, V. Buschmann, B. Krämer et al., Comparison and accuracy of methods to determine the confocal volume for quantitative fluorescence correlation spectroscopy, J. Microscopy 232, 343 (2008).
C.B. Müller, K. Weiß, W. Richtering, A. Loman, J. Enderlein, Calibrating Differential Interference Contrast Microscopy with dual-focus Fluorescence Correlation Spectroscopy, Optics Express 16, 4322 (2008).
T. Sanden, G. Persson, J. Widengren, Transient State Imaging for Microenvironmental Monitoring by Laser Scanning Microscopy, Anal. Chem. 80, 9589 (2008).
E. Kalie, D.A. Jaitin, Y. Podoplelova, J. Piehler, G. Schreiber, The Stability of the Ternary Interferon-Receptor Complex Rather than the Affinity to the Individual Subunits Dictates Differential Biological Activities, J. Biol. Chem. 283, 32925 (2008).
T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann et al., Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements, ChemPhysChem 8, 433 (2007).
G. Zauner, E. Lonardi, L. Bubacco, T.J. Aartsma et al., Tryptophan to Dye Fluorescence Energy Transfer Applied to Oxygen Sensing by Using Type-3 Copper Proteins, Chem. Eur. J. 13, 7085 (2007).
N. Marmé, J.-P. Knemeyer, Sensitive Bioanalysis – combining single-molecule spectroscopy with mono-labeled self-quenching probes, Anal. Bioanal. Chem. 388, 1075 (2007).
A. Friedrich, J.D. Hoheisel, N. Marmé, J.-P. Knemeyer, DNA-probes fort he highly sensitive identification of single nucleotide polymorphism using single-molecule spectroscopy, FEBS Lett. 581, 1644 (2007).
K.-H. Roh, M. Yoshida, J. Lahann, Water-Stable Biphasic Nanocolloids with Potential Use as Anisotropic Imaging Probes, Langmuir 23, 5683 (2007).
T. Dertinger, I. von der Hocht, A. Benda, M. Hof, J. Enderlein, Surface Sticking and Lateral Diffusion of Lipids Supported Biolayers, Langmuir 22, 9339 (2006).
T. Dertinger, I. Gregor, I. von der Hocht, R. Erdmann, B. Krämer, F. Koberling, Measuring precise diffusion coefficients with two-focus fluorescence correlation spectroscopy, Proc. SPIE 6092, 609203 (2006).
C. Eggeling, J. Widengren, L. Brand, J. Schaffer et al., Analysis of Photobleaching in Single-Molecule Multicolor Excitation and Förster Resonance Energy Transfer Measurements, J. Phys. Chem. A 110, 2979 (2006).
J.P. Klare, E. Bordignon, M. Doebber, J. Fitter et al., Effects of Solubilization on the Structure and Function of the Sensory Rhodopsin II/Transducer Complex, J. Mol. Biol. 365, 1207 (2006).
A.E. Miller, A.J. Fischer, T. Laurence, Single-molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy, PNAS 103, 11136 (2006).
S. Doose, H. Neuweiler, M. Sauer, A close look at fluorescence quenching of organic dyes by tryptophan, ChemPhysChem 6, 2277 (2005).
K. Stöhr, B. Hafner, O. Nolte, J. Wolfrum, M. Sauer, D.-P. Herten, Species-Specific Identification of Mycobacterial 16S rRNA PCR Amplicons Using Smart Probes, Anal. Chem. 77, 7195 (2005).
F. Koberling, M. Wahl, M. Patting, H.-J. Rahn, P. Kapusta, R. Erdmann, Two channel fluorescence lifetime microscope with two colour laser excitation, single molecule sensitivity and sub-micrometer resolution, Proc. SPIE 5143, 181 (2003).