ATTO 665

ATTO 665

Optical Properties
λ_abs	662	nm
ε_max	1.60×10⁵	M^-1cm^-1
λ_fl	680	nm
η_fl	60	%
τ_fl	2.9	ns
CF₂₆₀ = ε₂₆₀/ε_max	0.07
CF₂₈₀ = ε₂₈₀/ε_max	0.06

ATTO 665 is a new fluorescent label related to ATTO 647N. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 665 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. ATTO 665 is a hydrophobic dye. The fluorescence is excited most efficiently in the range 640 - 675 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.

Documents

Product Sheets

- ATTO 665 product information

- NHS-ester
- maleimide
- phalloidin
- azide/alkyne
- streptavidin

Spectra

Absorption and Emission Spectrum (ASCII)

- ATTO 665 absorption (.txt)

- ATTO 665 emission (.txt)

Absorption and Emission Spectrum (grafic)

- ATTO 665 absorption/fluorescence (.jpg)

MSDS

Material Safety Data Sheets

- ATTO 665 carboxy MSDS

- ATTO 665 NHS-ester MSDS

- ATTO 665 maleimide MSDS

- ATTO 665 streptavidin MSDS

- ATTO 665 biotin MSDS

- ATTO 665 phalloidin MSDS

- ATTO 665 azide MSDS

Literature

Selected Publications for ATTO 665:

A. Gómez-Sánchez, R. Vitale, O. Devos, A. de Juan, C. Ruckebusch, Kernelizing, Analytica Chimica Acta 1273, 341545 (2023).

X. Chen, Y. Liu, X. Liu, C. Lu, Nanoparticle-based single molecule fluorescent probes, Luminescence 37, 1808 (2022).

S. Pramanik, S. Sreedharan, R. Tiwari, S. Dutta, N. Kandoth, S. Barman, S. Aderinto, S. Chattopadhyay, A. Das, J. Thomas, Nanoparticles for super-resolution microscopy, Chemical Society reviews 51, 9882 (2022).

U. Schimpf, E. Caldas-Silveira, L. Katchan, C. Vigier-Carriere, I. Lantier, G. Nachmann, S. Gidlöf, A. Jonasson, L. Björndahl, S. Trombotto, X. Druart, T. Crouzier, Topical reinforcement of the cervical mucus barrier to sperm, Science translational medicine 14, eabm2417 (2022).

N. Melnychuk, S. Egloff, A. Runser, A. Reisch, A. Klymchenko, Light‐Harvesting Nanoparticle Probes for FRET‐Based Detection of Oligonucleotides with Single‐Molecule Sensitivity, Angewandte Chemie International Edition 59, 6811 (2020).

S. Reinicke, T. Fischer, J. Bramski, J. Pietruszka, A. Böker, Biocatalytically active microgels by precipitation polymerization of N -isopropyl acrylamide in the presence of an enzyme, RSC Advances 9, 28377 (2019).

M. Raab, I. Jusuk, J. Molle, E. Buhr, B. Bodermann, D. Bergmann, H. Bosse, P. Tinnefeld, Using DNA origami nanorulers as traceable distance measurement standards and nanoscopic benchmark structures, Scientific Reports 8, 1780 (2018).

S. Dominguez-Medina, S. Chen, J. Blankenburg, P. Swanglap, C. Landes, S. Link, Measuring the Hydrodynamic Size of Nanoparticles Using Fluctuation Correlation Spectroscopy, Annual review of physical chemistry 67, 489 (2016).

S. Li, T. Zhang, Z. Zhu, N. Gao, Q.-H. Xu, Lighting up the gold nanoparticles quenched fluorescence by silver nanoparticles, RSC Advances 6, 58566 (2016).

Z. Petrášek, J. Wiedemann, P. Schwille, Towards a spectrum-based bar code for identification of weakly fluorescent microparticles, Methods and Applications in Fluorescence 2, 15004 (2014).

R. Doshi, H. van Veen, Substrate Binding Stabilizes a Pre-translocation Intermediate in the ATP-binding Cassette Transport Protein MsbA, Journal of Biological Chemistry 288, 21638 (2013).

J. Sloniec, U. Resch-Genger, A. Henning, Photophysics and Release Kinetics of Enzyme-Activatable Optical Probes Based on H Dimerized Fluorophores on Self-Immolative Linkers, J. Phys. Chem. B 117, 14336 (2013).

Product No.:: AD 665

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ATTO 665

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