ATTO 665
Optical Properties |
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λabs |
662 |
nm |
||
εmax |
1.60×105 |
M-1 cm-1 |
||
λfl |
680 |
nm |
||
ηfl |
60 |
% |
||
τfl |
2.9 |
ns |
||
CF260 = ε260/εmax |
0.07 |
|
||
CF280 = ε280/εmax |
0.06 |
|
ATTO 665 is a new fluorescent label related to ATTO 647N. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 665 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. ATTO 665 is a hydrophobic dye. The fluorescence is excited most efficiently in the range 640 - 675 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.