ATTO Rho11

ATTO Rho11

Optical Properties
λ_abs	572	nm
ε_max	1.2×10⁵	M^-1cm^-1
λ_fl	595	nm
η_fl	80	%
τ_fl	4.0	ns
CF₂₆₀ = ε₂₆₀/ε_max	0.26
CF₂₈₀ = ε₂₈₀/ε_max	0.10

ATTO Rho11 is a new rhodamine dye featuring a functionality for coupling to biomolecules such as DNA, RNA, proteins etc. The label shows very high fluorescence efficiency and exceptionally high photo-stability. The dye is moderately hydrophilic. ATTO Rho11 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1. In solution its fluorescence can be excited efficiently in the range 550 - 580 nm.

Documents

Product Sheets

- ATTO Rho11 product information

- NHS-ester
- maleimide
- phalloidin

Spectra

Absorption and Emission Spectrum (ASCII)

- ATTO Rho11 Absorption (.txt)

- ATTO Rho11 Emission (.txt)

Absorption and Emission Spectrum (graphic)

- ATTO Rho11 absorption/fluorescence (.jpg)

MSDS

Material Safety Data Sheets

- ATTO Rho11 carboxy MSDS

- ATTO Rho11 NHS-ester MSDS

- ATTO Rho11 maleimide MSDS

- ATTO Rho11 biotin MSDS

- ATTO Rho11 phalloidin MSDS

Literature

Selected Publications for ATTO Rho11:

J. Sotolongo Bellón, O. Birkholz, C. Richter, F. Eull, H. Kenneweg, S. Wilmes, U. Rothbauer, C. You, M. Walter, R. Kurre, J. Piehler, Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane, Cell Reports Methods 2, 100165 (2022).

C. Gorby, J. Sotolongo Bellón, S. Wilmes, W. Warda, E. Pohler, P. Fyfe, A. Cozzani, C. Ferrand, M. Walter, S. Mitra, J. Piehler, I. Moraga, Engineered IL-10 variants elicit potent immunomodulatory effects at low ligand doses, Science signaling 13 (2020).

C. Ho, A. Chhabra, P. Starkl, P.-J. Schnorr, S. Wilmes, I. Moraga, H.-S. Kwon, N. Gaudenzio, R. Sibilano, T. Wehrman, M. Gakovic, J. Sockolosky, M. Tiffany, A. Ring, J. Piehler, I. Weissman, S. Galli, J. Shizuru, K. Garcia, Decoupling the Functional Pleiotropy of Stem Cell Factor by Tuning c-Kit Signaling>, Cell 168, 1041-1052.e18 (2017).

A. Kim, J. Ulirsch, S. Wilmes, E. Unal, I. Moraga, M. Karakukcu, D. Yuan, S. Kazerounian, N. Abdulhay, D. King, N. Gupta, S. Gabriel, E. Lander, T. Patiroglu, A. Ozcan, M. Ozdemir, K. Garcia, J. Piehler, H. Gazda, D. Klein, V. Sankaran, Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation, Cell 168, 1053-1064.e15 (2017).

I. Moraga, G. Wernig, S. Wilmes, V. Gryshkova, C. Richter, W.-J. Hong, R. Sinha, F. Guo, H. Fabionar, T. Wehrman, P. Krutzik, S. Demharter, I. Plo, I. Weissman, P. Minary, R. Majeti, S. Constantinescu, J. Piehler, K. Garcia, Tuning cytokine receptor signaling by re-orienting dimer geometry with surrogate ligands, Cell 160, 1196 (2015).

Product No.:: AD Rho11

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