ATTO Rho14

ATTO Rho14

Optical Properties
λ_abs	625	nm
ε_max	1.4×10⁵	M^-1cm^-1
λ_fl	646	nm
η_fl	80	%
τ_fl	3.7	ns
CF₂₆₀ = ε₂₆₀/ε_max	0.29
CF₂₈₀ = ε₂₈₀/ε_max	0.46

ATTO Rho14 is a new rhodamine dye featuring a functionality for coupling to biomolecules such as DNA, RNA or proteins. The label shows strong absorption and extraordinarily high fluorescence quantum yield. In fact ATTO Rho14 is the brightest label available in this wavelength range. In addition the dye exhibits an exceptionally high photo-stability. In common with most ATTO-labels, absorption and fluorescence are pH-independent in the range of pH 2 to 11, used in typical applications. The dye is moderately hydrophilic. After coupling to a substrate ATTO Rho14 carries a net electrical charge of +1. The fluorescence is excited efficiently in the range 605 - 640 nm. A suitable excitation source for ATTO Rho14 is the 633 nm line of the He:Ne laser or a diode-laser emitting at 635 nm.

Documents

Product Sheets

ATTO Rho14 product information

- NHS-ester
- maleimide
- phalloidin

Spectra

Absorption and Emission Spectrum (ASCII)

- ATTO Rho14 Absorption (.txt)

- ATTO Rho14 Emission (.txt)

Absorption and Emission Spectrum (graphic)

- ATTO Rho14 absorption/fluorescence (.jpg)

MSDS

Material Safety Data Sheets

- ATTO Rho14 carboxy MSDS

- ATTO Rho14 NHS-ester MSDS

- ATTO Rho14 maleimide MSDS

- ATTO Rho14 biotin MSDS

- ATTO Rho14 phalloidin MSDS

Literature

Selected Publications for ATTO Rho14:

H. Xiong, J. Lee, R. Zare, W. Min, Strong Concentration Enhancement of Molecules at the Interface of Aqueous Microdroplets, The journal of physical chemistry. B 124, 9938 (2020).

S. Wen, J. Zhou, P. Schuck, Y. Doug Suh, T. Schmidt, D. Jin, Future and challenges for hybrid upconversion nanosystems, Nature Photonics 13, 828 (2019).

H. Xiong, N. Qian, Y. Miao, Z. Zhao, W. Min, Stimulated Raman Excited Fluorescence Spectroscopy of Visible Dyes, The Journal of Physical Chemistry Letters 10, 3563 (2019).

N. Ankenbruck, T. Courtney, Y. Naro, A. Deiters, Optochemical Control of Biological Processes in Cells and Animals, Angewandte Chemie International Edition 57, 2768 (2018).

U. Cho, D. Riordan, P. Ciepla, K. Kocherlakota, J. Chen, P. Harbury, Ultrasensitive optical imaging with lanthanide lumiphores, Nature chemical biology 14, 15 (2018).

I. Gaspar, F. Wippich, A. Ephrussi, Enzymatic production of single-molecule FISH and RNA capture probes, RNA 23, 1582 (2017).

M. Fichte, X. Weyel, S. Junek, F. Schäfer, C. Herbivo, M. Goeldner, A. Specht, J. Wachtveitl, A. Heckel, Three‐Dimensional Control of DNA Hybridization by Orthogonal Two‐Color Two‐Photon Uncaging, Angewandte Chemie 128, 9094 (2016).

J. Schumm, C. Gutierrez-Mateo, A 27-Locus STR Assay to Meet All United States and European Law Enforcement Agency Standards, J. Forensic Sci. 58, 1584 (2013).

W. Zou, C. Visser, J. Maduro, M. Pshenichnikov, J. Hummelen, Broadband dye-sensitized upconversion of near-infrared light, Nature Photonics 6, 560 (2012).

Product No.:: AD Rho14

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ATTO Rho14

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