Cyan 500 NHS-Ester
Optical Properties (in PBS, pH 7.4) |
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λabs |
450 |
nm |
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εmax |
4.5×104 |
M-1 cm-1 |
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λfl |
495 |
nm |
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ηfl |
85 |
% |
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CF260 = ε260/εmax |
0.31 |
|
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CF280 = ε280/εmax |
0.30 |
|
Cyan 500 NHS-Ester is an amine-reactive fluorescent label. It is designed and recommended by Roche for application in LightCycler® instruments. The dye is used to label amino-modified oligonucleotides which can be detected on excitation at 430 nm in Roche LightCycler® instruments.
Cyan 500 NHS-Ester can also be used as label for other biomolecules such as proteins etc. The dye has a molecular weight of 580 g/mol and is moderately hydrophilic.