ATTO 633
Optical Properties |
||||
λabs |
630 |
nm |
||
εmax |
1.3×105 |
M-1 cm-1 |
||
λfl |
651 |
nm |
||
ηfl |
64 |
% |
||
τfl |
3.3 |
ns |
||
CF260 = ε260/εmax |
0.04 |
|
||
CF280 = ε280/εmax |
0.05 |
|
ATTO 633 belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 633 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.
In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 633 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1. The label is moderately hydrophilic. Its fluorescence is excited most efficiently in the range 610 - 645 nm. A suitable excitation source for ATTO 633 is the 633 nm line of the He:Ne laser or a diode-laser emitting at 635 nm.
Product Sheets
- ATTO 633 product information
- NHS-ester
- maleimide
- iodoacetamide
- phalloidin
- amine
- azide/alkyne
- streptavidin
Absorption and Emission Spectrum (ASCII)
- ATTO 633 absorption (.txt)
- ATTO 633 emission (.txt)
Absorption and Emission Spectrum (graphic)
- ATTO 633 absorption/fluorescence (.jpg)
Material Safety Data Sheets
- ATTO 633 carboxy MSDS
- ATTO 633 NHS-ester MSDS
- ATTO 633 maleimide MSDS
- ATTO 633 streptavidin MSDS
- ATTO 633 biotin MSDS
- ATTO 633 phalloidin MSDS
- ATTO 633 amine MSDS
- ATTO 633 azide MSDS
- ATTO 633 hydrazide MSDS
- ATTO 633 iodoacetamide MSDS
- ATTO 633 alkyne MSDS
Selected Publications for ATTO 633:
M. Bao, J. Xie, Geometric Confinement-Mediated Mechanical Tension Directs Patterned Differentiation of Mouse ESCs into Organized Germ Layers, ACS Applied Materials & Interfaces 15, 34397 (2023).
P. Dave, G. Roth, E. Griesbach, D. Mateju, T. Hochstoeger, J. Chao, Single-molecule imaging reveals translation-dependent destabilization of mRNAs, Molecular Cell 83, 589-606.e6 (2023).
A. Di Costagliola Polidoro, Z. Baghbantarghdari, V. de Gregorio, S. Silvestri, P. Netti, E. Torino, Insulin Activation Mediated by Uptake Mechanisms, Biomacromolecules 24, 2203 (2023).
T. Gui, C. Fleming, C. Manzato, B. Bourgeois, N. Sirati, J. Heuer, I. Papadionysiou, D. van Montfort, M. van Gijzen, L. Smits, B. Burgering, T. Madl, J. Schuijers, Targeted perturbation of signaling-driven condensates, Molecular Cell 83, 4141-4157.e11 (2023).
I. Hefetz, O. Israeli, G. Bilinsky, I. Plaschkes, E. Hazkani-Covo, Z. Hayouka, A. Lampert, Y. Helman, A reversible mutation in a genomic hotspot saves bacterial swarms from extinction, iScience 26, 106043 (2023).
X. Jiang, J. Mietner, J. Navarro, A combination of surface-initiated controlled radical polymerization (SET-LRP) and click-chemistry for the chemical modification and fluorescent labeling of cellulose nanofibrils, Cellulose 30, 2929 (2023).
V. Karema-Jokinen, A. Koskela, M. Hytti, H. Hongisto, T. Viheriälä, M. Liukkonen, T. Torsti, H. Skottman, A. Kauppinen, S. Nymark, K. Kaarniranta, Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients, Journal of Biological Chemistry 299, 104770 (2023).
E. Krok, H. Franquelim, M. Chattopadhyay, H. Orlikowska-Rzeznik, P. Schwille, L. Piatkowski, Nanoscale structural response of biomimetic cell membranes to controlled dehydration, Nanoscale 16, 72 (2023).
E. Krok, M. Stephan, R. Dimova, L. Piatkowski, Tunable biomimetic bacterial membranes from binary and ternary lipid mixtures and their application in antimicrobial testing, Biochimica et Biophysica Acta (BBA) - Biomembranes 1865, 184194 (2023).
E. Mäki-Mikola, P. Lauren, N. Uema, K. Kikuchi, Y. Takashima, T. Laaksonen, T. Lajunen, Establishing a simple perfusion cell culture system for light-activated liposomes, Scientific reports 13, 2050 (2023).
A. Mirza, M. Akhtar, J. Aguren, J. Marino, J. Bruno, Advancements in Rapid and Affordable Diagnostic Testing for Respiratory Infectious Diseases, Journal of fluorescence (2023).
P. Perez Schmidt, T. Luedtke, P. Moretti, P. Di Gianvincenzo, M. Fernandez Leyes, B. Espuche, H. Amenitsch, G. Wang, H. Ritacco, L. Polito, M. Ortore, S. Moya, Assembly and recognition mechanisms of glycosylated PEGylated polyallylamine phosphate nanoparticles, Journal of Colloid and Interface Science 645, 448 (2023).
K. Riedlová, M. Saija, A. Olżyńska, P. Jurkiewicz, P. Daull, J.-S. Garrigue, L. Cwiklik, Influence of BAKs on tear film lipid layer, European Journal of Pharmaceutics and Biopharmaceutics 186, 65 (2023).
R. Sahu, C. Goswami, Presence of TRPV3 in macrophage lysosomes helps in skin wound healing against bacterial infection, Experimental Dermatology 32, 60 (2023).
R. West, K. Kanellopulos, S. Schmid, Photothermal Microscopy and Spectroscopy with Nanomechanical Resonators, The Journal of Physical Chemistry C 127, 21915 (2023).
L. Cassella, A. Ephrussi, Subcellular spatial transcriptomics identifies three mechanistically different classes of localizing RNAs, Nature Communications 13, 1 (2022).
S. Dey, A. Dorey, L. Abraham, Y. Xing, I. Zhang, F. Zhang, S. Howorka, H. Yan, A reversibly gated protein-transporting membrane channel made of DNA, Nature Communications 13, 1 (2022).
J. Dodge, A. Doyle, A. Costa-da-Silva, C. Hogden, E. Mezey, J. Mays, Atto 465 Derivative Is a Nuclear Stain with Unique Excitation and Emission Spectra Useful for Multiplex Immunofluorescence Histochemistry, Journal of Histochemistry & Cytochemistry 70, 211 (2022).
V. Kast, A. Nadernezhad, D. Pette, A. Gabrielyan, M. Fusenig, K. Honselmann, D. Stange, C. Werner, D. Loessner, A Tumor Microenvironment Model of Pancreatic Cancer to Elucidate Responses toward Immunotherapy, Advanced Healthcare Materials n/a, 2201907 (2022).
M. Klein, K. Shulenberger, U. Barotov, T. Šverko, M. Bawendi, Supramolecular Lattice Deformation and Exciton Trapping in Nanotubular J-Aggregates, The Journal of Physical Chemistry C 126, 4095 (2022).
E. Krok, A. Batura, M. Chattopadhyay, H. Orlikowska, L. Piatkowski, Lateral organization of biomimetic cell membranes in varying pH conditions, Journal of Molecular Liquids 345, 117907 (2022).
N. Louros, M. Ramakers, E. Michiels, K. Konstantoulea, C. Morelli, T. Garcia, N. Moonen, S. D’Haeyer, V. Goossens, D. Thal, D. Audenaert, F. Rousseau, J. Schymkowitz, Mapping the sequence specificity of heterotypic amyloid interactions enables the identification of aggregation modifiers, Nature Communications 13, 1 (2022).
N. Mirzazadeh Dizaji, Y. Lin, T. Bein, E. Wagner, S. Wuttke, U. Lächelt, H. Engelke, Biomimetic Mineralization of Iron-Fumarate Nanoparticles for Protective Encapsulation and Intracellular Delivery of Proteins, Chemistry of materials : a publication of the American Chemical Society 34, 8684 (2022).
M. Nguyen, D. Biriukov, C. Tempra, K. Baxova, H. Martinez-Seara, H. Evci, V. Singh, R. Šachl, M. Hof, P. Jungwirth, M. Javanainen, M. Vazdar, Ionic Strength and Solution Composition Dictate the Adsorption of Cell-Penetrating Peptides onto Phosphatidylcholine Membranes, Langmuir : the ACS journal of surfaces and colloids 38, 11284 (2022).
N. Niranjan Dhanasekar, D. Thiyagarajan, D. Bhatia, DNA Origami in the Quest for Membrane Piercing, Chemistry – An Asian Journal 17, e202200591 (2022).
S. Pritzl, D. Konrad, M. Ober, A. Richter, J. Frank, B. Nickel, D. Trauner, T. Lohmüller, Optical Membrane Control with Red Light Enabled by Red-Shifted Photolipids, Langmuir 38, 385 (2022).
S. Pritzl, J. Morstein, S. Kahler, D. Konrad, D. Trauner, T. Lohmüller, Postsynthetic Photocontrol of Giant Liposomes via Fusion-Based Photolipid Doping, Langmuir : the ACS journal of surfaces and colloids 38, 11941 (2022).
P. Ramamurthi, Z. Zhao, E. Burke, N. Steinmetz, M. Müllner, Tuning the Hydrophilic–Hydrophobic Balance of Molecular Polymer Bottlebrushes Enhances their Tumor Homing Properties, Advanced Healthcare Materials 11, 2200163 (2022).
P. Sutthavas, Z. Tahmasebi Birgani, P. Habibovic, S. van Rijt, Calcium Phosphate-Coated and Strontium-Incorporated Mesoporous Silica Nanoparticles Can Effectively Induce Osteogenic Stem Cell Differentiation, Advanced Healthcare Materials 11, 2101588 (2022).
H. Varol, S. Seeger, Fluorescent Staining of Silicone Micro- and Nanopatterns for Their Optical Imaging, Langmuir 38, 231 (2022).
Z. Zhao, D. Roy, J. Steinkühler, T. Robinson, R. Lipowsky, R. Dimova, Super-Resolution Imaging of Highly Curved Membrane Structures in Giant Vesicles Encapsulating Molecular Condensates, Advanced Materials 34, 2106633 (2022).
T. Altman, A. Ionescu, A. Ibraheem, D. Priesmann, T. Gradus-Pery, L. Farberov, G. Alexandra, N. Shelestovich, R. Dafinca, N. Shomron, F. Rage, K. Talbot, M. Ward, A. Dori, M. Krüger, E. Perlson, Axonal TDP-43 condensates drive neuromuscular junction disruption through inhibition of local synthesis of nuclear encoded mitochondrial proteins, Nature Communications 12, 1 (2021).
J. Auerswald, J. Ebenhan, C. Schwieger, A. Scrima, A. Meister, K. Bacia, Measuring protein insertion areas in lipid monolayers by fluorescence correlation spectroscopy, Biophysical Journal 120 (2021).
B. Benham-Pyle, C. Brewster, A. Kent, F. Mann, S. Chen, A. Scott, A. Box, A. Sánchez Alvarado, Identification of rare, transient post-mitotic cell states that are induced by injury and required for whole-body regeneration in Schmidtea mediterranea, Nature Cell Biology 23, 939 (2021).
J. Burke, A. Gilchrist, S. Sawyer, R. Parker, RNase L limits host and viral protein synthesis via inhibition of mRNA export, Science Advances 7, 120 (2021).
M. Chattopadhyay, E. Krok, H. Orlikowska, P. Schwille, H. Franquelim, L. Piatkowski, Hydration Layer of Only a Few Molecules Controls Lipid Mobility in Biomimetic Membranes, Journal of the American Chemical Society 143, 14551 (2021).
M. Klein, K. Shulenberger, U. Barotov, T. Šverko, M. Bawendi, Supramolecular Lattice Deformation and Exciton Trapping in Nanotubular J-Aggregates, The Journal of Physical Chemistry C 126, 4095 (2022).
J. Lee, K. Lee, C. Wang, D. Ha, G.-H. Kim, J. Park, T. Kim, Combined Effects of Zeta-potential and Temperature of Nanopores on Diffusioosmotic Ion Transport, Analytical Chemistry 93, 14169 (2021).
R. Leisi, E. Widmer, B. Gooch, N. Roth, C. Ros, Mechanistic insights into flow-dependent virus retention in different nanofilter membranes, Journal of Membrane Science 636, 119548 (2021).
A. Niederberger, T. Pelras, L. Manni, P. FitzGerald, G. Warr, M. Müllner, Stiffness-Dependent Intracellular Location of Cylindrical Polymer Brushes, Macromolecular Rapid Communications 42, 2100138 (2021).
M. Partipilo, E. Ewins, J. Frallicciardi, T. Robinson, B. Poolman, D. Slotboom, Minimal Pathway for the Regeneration of Redox Cofactors, JACS Au 1, 2280 (2021).
S. Safarian, P. Kusov, S. Kosolobov, O. Borzenkova, A. Khakimov, Y. Kotelevtsev, V. Drachev, Surface-specific washing-free immunosensor for time-resolved cortisol monitoring, Talanta 225, 122070 (2021).
S. Suarasan, C. Tira, M. Rusu, A.-M. Craciun, M. Focsan, Controlled fluorescence manipulation by core-shell multilayer of spherical gold nanoparticles, Journal of Molecular Structure 1244, 130950 (2021).
H. Ucar, H.-A. Wagenknecht, DNA-templated control of chirality and efficient energy transport in supramolecular DNA architectures with aggregation-induced emission, Chemical Science 12, 10048 (2021).
J. Xie, M. Bao, X. Hu, W. Koopman, W. Huck, Energy expenditure during cell spreading influences the cellular response to matrix stiffness, Biomaterials 267, 120494 (2021).
Y. Xue, I. Braslavsky, S. Quake, Temperature effect on polymerase fidelity, Journal of Biological Chemistry 297, 101270 (2021).
A. Zengin, J. Castro, P. Habibovic, S. van Rijt, Injectable, self-healing mesoporous silica nanocomposite hydrogels with improved mechanical properties, Nanoscale 13, 1144 (2021).
S. Azinheiro, K. Kant, M.-A. Shahbazi, A. Garrido-Maestu, M. Prado, L. Dieguez, A smart microfluidic platform for rapid multiplexed detection of foodborne pathogens, Food Control 114, 107242 (2020).
D. Bölükbas, S. Datz, C. Meyer-Schwickerath, C. Morrone, A. Doryab, D. Gößl, M. Vreka, L. Yang, C. Argyo, S. van Rijt, M. Lindner, O. Eickelberg, T. Stoeger, O. Schmid, S. Lindstedt, G. Stathopoulos, T. Bein, D. Wagner, S. Meiners, Organ-Restricted Vascular Delivery of Nanoparticles for Lung Cancer Therapy, Advanced Therapeutics 3, 2000017 (2020).
D. Boytsov, C. Hannesschlaeger, A. Horner, C. Siligan, P. Pohl, Micropipette Aspiration-Based Assessment of Single Channel Water Permeability, Biotechnology Journal 15, 1900450 (2020).
L. Chen, X. Chen, X. Yang, C. He, M. Wang, P. Xi, J. Gao, Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences, Computational and Structural Biotechnology Journal 18, 2209 (2020).
A. Milewska, K. Owczarek, A. Szczepanski, K. Pyrc, Visualizing Coronavirus Entry into Cells, Methods in molecular biology (Clifton, N.J.) 2203, 241 (2020).
A. Olżyńska, A. Wizert, M. Štefl, D. Iskander, L. Cwiklik, Mixed polar-nonpolar lipid films as minimalistic models of Tear Film Lipid Layer, Biochimica et Biophysica Acta (BBA) - Biomembranes 1862, 183300 (2020).
M. Reichardt, C. Neuhaus, J.-D. Nicolas, M. Bernhardt, K. Toischer, T. Salditt, X-Ray Structural Analysis of Single Adult Cardiomyocytes, Biophysical Journal 119, 1309 (2020).
M. Sabatino, F. Carfì Pavia, S. Rigogliuso, D. Giacomazza, G. Ghersi, V. La Carrubba, C. Dispenza, Development of injectable and durable kefiran hydro-alcoholic gels, International Journal of Biological Macromolecules 149, 309 (2020).
M. Sabatino, L. Ditta, A. Conigliaro, C. Dispenza, A multifuctional nanoplatform for drug targeted delivery based on radiation-engineered nanogels, Radiation Physics and Chemistry 169, 108059 (2020).
R. Šachl, S. Čujová, V. Singh, P. Riegerová, P. Kapusta, H.-M. Müller, J. Steringer, M. Hof, W. Nickel, Functional Assay to Correlate Protein Oligomerization States with Membrane Pore Formation, Analytical Chemistry 92, 14861 (2020).
J. Hu, S. Gupta, J. Ozdemir, M. Beyzavi, Applications of Dynamic Covalent Chemistry Concept toward Tailored Covalent Organic Framework Nanomaterials, ACS Applied Nano Materials 3, 6239 (2020).
N. Larkey, J. Phillips, H. Jang, S. Kolluri, S. Burrows, Small RNA Biosensor Design Strategy To Mitigate Off-Analyte Response, ACS Sensors 5, 377 (2020).
L. Andrée, D. Barata, P. Sutthavas, P. Habibovic, S. van Rijt, Guiding mesenchymal stem cell differentiation using mesoporous silica nanoparticle-based films, Acta Biomaterialia 96, 557 (2019).
N. Garino, T. Limongi, B. Dumontel, M. Canta, L. Racca, M. Laurenti, M. Castellino, A. Casu, A. Falqui, V. Cauda, A Microwave-Assisted Synthesis of Zinc Oxide Nanocrystals Finely Tuned for Biological Applications, Nanomaterials 9, 212 (2019).
T. Godjevargova, Y. Ivanov, M. Atanasova, Z. Becheva, A. Zherdev, Magnetic nanoparticles based fluorescence immunoassay for food contaminants, Food Science and Applied Biotechnology 2, 38 (2019).
B. Hampoelz, A. Schwarz, P. Ronchi, H. Bragulat-Teixidor, C. Tischer, I. Gaspar, A. Ephrussi, Y. Schwab, M. Beck, Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis, Cell 179, 671-686.e17 (2019).
J.-D. Nicolas, M. Bernhardt, S. Schlick, M. Tiburcy, W.-H. Zimmermann, A. Khan, A. Markus, F. Alves, K. Toischer, T. Salditt, X-ray diffraction imaging of cardiac cells and tissue, Progress in Biophysics and Molecular Biology 144, 151 (2019).
P. Picone, M. Sabatino, A. Ajovalasit, D. Giacomazza, C. Dispenza, M. Di Carlo, Biocompatibility, hemocompatibility and antimicrobial properties of xyloglucan-based hydrogel film for wound healing application, International Journal of Biological Macromolecules 121, 784 (2019).
B. Ramesh, S. Abnouf, S. Mali, W. Moree, U. Patil, S. Bark, N. Varadarajan, Engineered ChymotrypsiN for Mass Spectrometry-Based Detection of Protein Glycosylation, ACS Chemical Biology 14, 2616 (2019).
K. Sy Piecco, J. Vicente, J. Pyle, D. Ingram, M. Kordesch, J. Chen, Reusable Chemically Micropatterned Substrates via Sequential Photoinitiated Thiol–Ene Reactions as a Template for Perovskite Thin-Film Microarrays, ACS Applied Electronic Materials 1, 2279 (2019).
R. Thomsen, M. Galsgaard Malle, A. Hauge Okholm, S. Krishnan, S. Bohr, R. Schøler Sørensen, O. Ries, S. Vogel, F. Simmel, N. Hatzakis, J. Kjems, A large size-selective DNA nanopore with sensing applications, Nature Communications 10, 1 (2019).
S. Wagle, V. Georgiev, T. Robinson, R. Dimova, R. Lipowsky, A. Grafmüller, Interaction of SNARE Mimetic Peptides with Lipid bilayers, Scientific Reports 9, 7708 (2019).
O. Wahlsten, F. Ulander, D. Midtvedt, M. Henningson, V. Zhdanov, B. Agnarsson, F. Höök, Quantitative Detection of Biological Nanoparticles in Solution via Their Mediation of Colocalization of Fluorescent Liposomes, Physical Review Applied 12 (2019).
P. Chidchob, D. Offenbartl-Stiegert, D. McCarthy, X. Luo, J. Li, S. Howorka, H. Sleiman, Spatial Presentation of Cholesterol Units on a DNA Cube as a Determinant of Membrane Protein-Mimicking Functions, Journal of the American Chemical Society 141, 1100 (2019).
S. Krause, T. Vosch, Stokes shift microscopy by excitation and emission imaging, Optics Express 27, 8208 (2019).
H. Xiong, N. Qian, Y. Miao, Z. Zhao, W. Min, Stimulated Raman Excited Fluorescence Spectroscopy of Visible Dyes, The Journal of Physical Chemistry Letters 10, 3563 (2019).
L. Avila, R. Chandrasekar, K. Wilkinson, J. Balthazor, M. Heerman, J. Bechard, S. Brown, Y. Park, G. Reek, J. Tomich, Delivery of lethal dsRNAs in insect diets by branched amphiphilic peptide capsules, Journal of Controlled Release 273, 139 (2018).
M. Bernhardt, J.-D. Nicolas, M. Osterhoff, H. Mittelstädt, M. Reuss, B. Harke, A. Wittmeier, M. Sprung, S. Köster, T. Salditt, Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy, Nature Communications 9, 3641 (2018).
E. Braselmann, A. Wierzba, J. Polaski, M. Chromiński, Z. Holmes, S.-T. Hung, D. Batan, J. Wheeler, R. Parker, R. Jimenez, D. Gryko, R. Batey, A. Palmer, A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells, Nature chemical biology 14, 964 (2018).
F. Fenaroli, U. Repnik, Y. Xu, K. Johann, S. van Herck, P. Dey, F. Skjeldal, D. Frei, S. Bagherifam, A. Kocere, R. Haag, B. de Geest, M. Barz, D. Russell, G. Griffiths, Enhanced Permeability and Retention-like Extravasation of Nanoparticles from the Vasculature into Tuberculosis Granulomas in Zebrafish and Mouse Models, ACS Nano 12, 8646 (2018).
E. Horak, R. Goldsmith, Drumming up single-molecule beats, Proceedings of the National Academy of Sciences 115, 11115 (2018).
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M. Jbeily, J. Kressler, Fluorophilicity and lipophilicity of fluorinated rhodamines determined by their partition coefficients in biphasic solvent systems, Journal of Fluorine Chemistry 193, 67 (2017).
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K. Grußmayer, D.-P. Herten, Time-resolved molecule counting by photon statistics across the visible spectrum, Physical chemistry chemical physics : PCCP 19, 8962 (2017).
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J. Qin, D. Zhao, S. Luo, W. Wang, J. Lu, M. Qiu, Q. Li, Strongly enhanced molecular fluorescence with ultra-thin optical magnetic mirror metasurfaces, Optics letters 42, 4478 (2017).
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F. Möller, M. Kieß, D. Braun, Photochemical Microscale Electrophoresis Allows Fast Quantification of Biomolecule Binding, Journal of the American Chemical Society 138, 5363 (2016).
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M. Li, S. Jorgensen, D. McMillan, Ł. Krzemiński, N. Daskalakis, R. Partanen, M. Tutkus, R. Tuma, D. Stamou, N. Hatzkas, L. Jeuken, Single Enzyme Experiments Reveal a Long-Lifetime Proton Leak State in a Heme-Copper Oxidase, Journal of the American Chemical Society 137, 16055 (2015).
S. Little, K. Sinsimer, J. Lee, E. Wieschaus, E. Gavis, Independent and coordinate trafficking of single Drosophila germ plasm mRNAs, Nature cell biology 17, 558 (2015).
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